https://scholars.lib.ntu.edu.tw/handle/123456789/406748
標題: | Kinetic studies of the oxidation of glutathione in protein refolding buffer | 作者: | Wang S.S.-S. Hung Y.-T. Lin Y.-C. |
關鍵字: | Glutathione;Kinetics;Oxidation;Refolding buffer;Size exclusion chromatography | 公開日期: | 2010 | 卷: | 33 | 期: | 2 | 起(迄)頁: | 277-286 | 來源出版物: | Bioprocess and Biosystems Engineering | 摘要: | Here, we examined the change of glutathione (GSH) under different conditions and determined the appropriate kinetic schemes to describe its change of concentration. GSH was continuously oxidized into glutathione disulfide (GSSG) as incubation period increased at the temperatures ranging from 10 to 50 ¢XC while certain oxidation by-products were also observed at the later stage of reaction at higher temperatures (70-90 ¢XC). The addition of 0.3 mM GSSG in 3 mM GSH solution delayed the onset of GSH oxidation without significantly changing the rate of GSH oxidation. Our results also revealed that GSH oxidation could be facilitated upon the addition of copper (II) ion whereas GSH oxidation was found to be decelerated when EDTA was present. In kinetic analysis, the reaction orders of GSH oxidation under various conditions were determined. Moreover, the temperature dependence of the rate of GSH oxidation was modeled by Arrhenius and Eyring equations and resultant activation parameters were also presented. ? 2009 Springer-Verlag. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/406748 | ISSN: | 16157591 | DOI: | 10.1007/s00449-009-0322-0 |
顯示於: | 化學工程學系 |
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