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  1. NTU Scholars
  2. 生物資源暨農學院
  3. 動物科學技術學系
Please use this identifier to cite or link to this item: https://scholars.lib.ntu.edu.tw/handle/123456789/406901
Title: A nutritional nonalcoholic steatohepatitis minipig model
Authors: Li, S.-J.
Ding, S.-T. 
Mersmann, H.J.
Chu, C.-H.
Hsu, C.-D.
Chen, C.-Y. 
Keywords: Autophagy; ER stress; IL-6; Lee-Sung minipigs; NAFLD; SAA
Issue Date: 2016
Journal Volume: 28
Start page/Pages: 51-60
Source: Journal of Nutritional Biochemistry 
Abstract: 
Background and Aims: The objective of this study was to elucidate whether a Western diet was associated with nonalcoholic steatohepatitis (NASH), and the relationship between NASH, autophagy and endoplasmic reticulum (ER) stress. Methods: Four-month-old Lee-Sung minipigs were randomly assigned to two groups: control diet (C) and Western diet (W), for a 5-month experimental period. Results: Feeding a Western diet produced a body composition with more fat, less lean and a greater liver weight. Compared with C pigs, W pigs also exhibited an elevated level of plasma insulin and free fatty acid. The W pigs displayed glucose intolerance, lower circulation antioxidant capacity and greater hepatic oxidative stress. Furthermore, pig fed the W diets had increased collagen accumulation in the liver and elevated systemic inflammation [tumor necrosis factor α and interleukin (IL)-6]. Compared with C pigs, W pigs had higher hepatic ER stress-related protein expression of GRP94, CHOP and caspase-12. The W pigs also had greater hepatic autophagy-related protein expression of p62 and LC3II. In an obesity antibody array analysis, W pigs had higher type 2 diabetes mellitus- (insulin-like growth factor 1, osteoprotegerin and resistin), atherosclerosis- (vascular endothelial growth factor, platelet-derived growth factor-AA and plasminogen activator inhibitor-I) and inflammation- [IL-1, macrophage-stimulating protein alpha, X-linked ectodermal dysplasia receptor and serum amyloid A (SAA)] related protein expressions. In addition, W pigs had greater plasma SAA concentration than C pigs and plasma SAA level was highly associated with IL-6. Conclusions: We successfully established a NASH pig model, and our findings suggested an association of NASH with ER stress and autophagy. The SAA has potential as a novel plasma biomarker for nonalcoholic fatty liver disease pigs. ? 2015 Elsevier Inc.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84957803888&doi=10.1016%2fj.jnutbio.2015.09.029&partnerID=40&md5=08aa67fbb4738b900279513983847f3a
https://scholars.lib.ntu.edu.tw/handle/123456789/406901
DOI: 10.1016/j.jnutbio.2015.09.029
SDG/Keyword: collagen; fatty acid; glucose regulated protein 94; insulin; interleukin 1; interleukin 6; macrophage activating factor; macrophage stimulating protein alpha; osteoprotegerin; plasminogen activator inhibitor; platelet derived growth factor AA; receptor; resistin; serum amyloid A; somatomedin C; thiobarbituric acid reactive substance; tumor necrosis factor alpha; unclassified drug; vasculotropin; x linked ectodermal dysplasia receptor; animal experiment; animal model; animal tissue; antioxidant activity; Article; atherosclerosis; autophagy; body composition; body fat; controlled study; diet induced obesity; endoplasmic reticulum stress; fatty acid blood level; glucose intolerance; inflammation; insulin blood level; insulin resistance; lean body weight; liver fibrosis; minipig; non insulin dependent diabetes mellitus; nonalcoholic fatty liver; nonhuman; oxidative stress; porcine model; protein blood level; protein expression; regulatory mechanism; Western diet; animal; disease model; endoplasmic reticulum stress; minipig; nonalcoholic fatty liver; pathology; pig; Animals; Body Composition; Disease Models, Animal; Endoplasmic Reticulum Stress; Non-alcoholic Fatty Liver Disease; Swine; Swine, Miniature
[SDGs]SDG3
Appears in Collections:動物科學技術學系

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