Manipulating Cellular Activities Using an Ultrasound-Chemical Hybrid Tool
Journal
ACS Synthetic Biology
Journal Volume
6
Journal Issue
11
Pages
2021-2027
Date Issued
2017
Author(s)
Fan, C.-H.
Huang, Y.-S.
Huang, W.-E.
Lee, A.A.
Ho, S.-Y.
Kao, Y.-L.
Wang, C.-L.
Lian, Y.-L.
Ueno, T.
Yeh, C.-K.
Lin, Y.-C.
Abstract
We developed an ultrasound-chemical hybrid tool to precisely manipulate cellular activities. A focused ultrasound coupled with gas-filled microbubbles was used to rapidly trigger the influx of membrane-impermeable chemical dimerizers into living cells to regulate protein dimerization and location without inducing noticeable toxicity. With this system, we demonstrated the successful modulation of phospholipid metabolism triggered by a short pulse of ultrasound exposure. Our technique offers a powerful and versatile tool for using ultrasound to spatiotemporally manipulate the cellular physiology in living cells.
SDGs
Type
journal article