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  4. Phase segregation of polymerizable lipids to construct filters for separating lipid-membrane-embedded species
 
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Phase segregation of polymerizable lipids to construct filters for separating lipid-membrane-embedded species

Journal
Biomicrofluidics
Journal Volume
8
Journal Issue
5
Date Issued
2014
Author(s)
Hu S.-K.
Chen Y.-M.
Chao L.  
DOI
10.1063/1.4895570
URI
https://scholars.lib.ntu.edu.tw/handle/123456789/410664
URL
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907192195&doi=10.1063%2f1.4895570&partnerID=40&md5=104ff4df1cf668baaa2d5a2a4e4bf26e
Abstract
Supported lipid bilayer (SLB) platforms have been developed to transport and separate membrane-embedded species in the species' native bilayer environment. In this study, we used the phase segregation phenomenon of lipid mixtures containing a polymerizable diacetylene phospholipid, 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DiynePC), and a nonpolymerizable phospholipid, 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), to create filter barrier structures in SLBs. Upon exposing the phase segregated samples to UV light, the DiynePC-rich domains could become crosslinked and remain fixed on the surface of the support, while the DOPC-rich regions, where no crosslinking could happen, could be removed later by detergent washing, and thus became the void regions in the filter. During the filter fabrication process, we used the laminar flow configuration in a microfluidic channel to control the spatial locations of the feed region and filter region in the SLB. The flow in a microfluidic channel was also used to apply a strong hydrodynamic shear stress to the SLB to transport the membrane-embedded species from the feed region to the filter region. We varied the DiynePC/DOPC molar ratio from 60/40 to 80/20 to adjust the cutoff size of the filter barriers and used two model membrane-embedded species of different sizes to examine the filtering capability. One of the model species, Texas Red 1,2-dihexa-decanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (Texas Red DHPE), had a single-lipid size, and the other species, cholera toxin subunit B-GM1 complex, had a multilipid size. When the DiynePC/DOPC molar ratio was 60/40, both species had high penetration ratios in the filter region. However, when the ratio was increased to 70/30, only the Texas Red DHPE, which was the smaller of the two model species, could penetrate the filter to a considerable extent. When the ratio was increased to 80/20, neither of the model species could penetrate the filter region. The results showed the possibility of using phase segregation of a mixture containing a polymerizable lipid and a nonpolymerizable lipid to fabricate filter barrier structures with tunable cutoff sizes in SLBs. ? 2014 AIP Publishing LLC.
SDGs

[SDGs]SDG3

Other Subjects
Bandpass filters; Fluidic devices; Laminar flow; Lipid bilayers; Microfluidics; Mixtures; Phase separation; Phospholipids; Polymers; Shear flow; Shear stress; 1 ,2-dioleoyl-sn-glycero-3-phosphocholine; Barrier structures; Filter fabrication; Flow configurations; Hydrodynamic shear stress; Microfluidic channel; Phase segregations; Supported lipid bilayer (SLB); Filtration
Type
journal article

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