https://scholars.lib.ntu.edu.tw/handle/123456789/410981
標題: | A Novel Thermal Bubble Valve Integrated Nanofluidic Preconcentrator for Highly Sensitive Biomarker Detection | 作者: | Deng C.-Z. Fan Y.-J. Chung P.-S. Sheen H.-J |
關鍵字: | acoustic wave; Brownian motion; C-reactive protein; immunobeads; nanofluidic preconcentrator; oscillating thermal microbubble | 公開日期: | 2018 | 出版社: | American Chemical Society | 卷: | 3 | 期: | 7 | 起(迄)頁: | 1409-1415 | 來源出版物: | ACS Sensors | 摘要: | In this study, we developed a new immunosensor that can achieve an ultralow detection limit and high sensitivity. This new device has an electrokinetic trapping (EKT)-based nanofluidic preconcentrator, which was integrated with oscillating bubble valves, to trap concentrated antigen and immunobeads. During the immunoassay process, oscillating bubbles rapidly grew and acted as control valves and to block the microchannel. Thereafter, the trapped preconcentrated antigen plug and antibody-coated nanobeads were preserved in the region between these two valves. Finally, the antigen concentration was quantitatively analyzed by a real-time measurement of Brownian diffusion of the immunobeads. In this work, the test sample used was C-reactive protein (CRP) which is a risk indicator of coronary heart disease and atherosclerosis. ? 2018 American Chemical Society. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048512121&doi=10.1021%2facssensors.8b00323&partnerID=40&md5=5a95013eb8c32535cd4581f0d3af656e https://scholars.lib.ntu.edu.tw/handle/123456789/410981 |
ISSN: | 23793694 | DOI: | 10.1021/acssensors.8b00323 | SDG/關鍵字: | Diseases; Nanofluidics; Brownian diffusion; C-reactive proteins; Coronary heart disease; Electrokinetic trapping; Low detection limit; Oscillating bubbles; Pre-concentrator; Real time measurements; Antigens; C reactive protein; immobilized antibody; chemistry; devices; diffusion; equipment design; human; immunoassay; lab on a chip; microfluidic analysis; sound; Antibodies, Immobilized; C-Reactive Protein; Diffusion; Equipment Design; Humans; Immunoassay; Lab-On-A-Chip Devices; Microfluidic Analytical Techniques; Sound |
顯示於: | 應用力學研究所 |
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