https://scholars.lib.ntu.edu.tw/handle/123456789/413276
標題: | Biosynthesis of Antroquinonol & 4-Acetylantroquinonol B via a Polyketide pathway using orsellinic acid as a ring precursor in Antrodia cinnamomea | 作者: | Chou K.C.-C. Yang S.-H. Wu H.-L. Lin P.-Y. Chang T.-L. Sheu F. Chen K.-H. FUU SHEU |
關鍵字: | 4-acetylantroquinonol B;Antrodia cinnamomea;Antroquinonol;Orsellinic acid;Polyketide pathway | 公開日期: | 2017 | 卷: | 65 | 期: | 1 | 起(迄)頁: | 74-86 | 來源出版物: | Journal of Agricultural and Food Chemistry | 摘要: | Antroquinonol (AQ) and 4-acetylantroquinonol B (4-AAQB), isolated from the mycelium of Antrodia cinnamomea, have a similar chemical backbone to coenzyme Q (CoQ). Based on the postulation that biosynthesis of both AQ and 4-AAQB in A. cinnamomea starts from the polyketide pathway, we cultivated this fungus in a culture medium containing [U-13C]oleic acid, and then we analyzed the crude extracts of the mycelium using UHPLC-MS. We found that AQ and 4-AAQB follow similar biosynthetic sequences as CoQ. Obvious [13C2] fragments on the ring backbone were detected in the mass spectrum for [13C2]AQ, [13C2]4-AAQB, and their [13C2] intermediates found in this study. The orsellinic acid, formed from acetyl-CoA and malonyl-CoA via the polyketide pathway, was found to be a novel benzoquinone ring precursor for AQ and 4- AAQB. The identification of endogenously synthesized farnesylated intermediates allows us to postulate the routes of AQ and 4- AAQB biosynthesis in A. cinnamomea. ? 2016 American Chemical Society. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/413276 | ISSN: | 00218561 | DOI: | 10.1021/acs.jafc.6b04346 |
顯示於: | 食品科技研究所 |
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