|Title:||Biosynthesis of Antroquinonol & 4-Acetylantroquinonol B via a Polyketide pathway using orsellinic acid as a ring precursor in Antrodia cinnamomea||Authors:||Chou K.C.-C.
|Keywords:||4-acetylantroquinonol B;Antrodia cinnamomea;Antroquinonol;Orsellinic acid;Polyketide pathway||Issue Date:||2017||Journal Volume:||65||Journal Issue:||1||Start page/Pages:||74-86||Source:||Journal of Agricultural and Food Chemistry||Abstract:||
Antroquinonol (AQ) and 4-acetylantroquinonol B (4-AAQB), isolated from the mycelium of Antrodia cinnamomea, have a similar chemical backbone to coenzyme Q (CoQ). Based on the postulation that biosynthesis of both AQ and 4-AAQB in A. cinnamomea starts from the polyketide pathway, we cultivated this fungus in a culture medium containing [U-13C]oleic acid, and then we analyzed the crude extracts of the mycelium using UHPLC-MS. We found that AQ and 4-AAQB follow similar biosynthetic sequences as CoQ. Obvious [13C2] fragments on the ring backbone were detected in the mass spectrum for [13C2]AQ, [13C2]4-AAQB, and their [13C2] intermediates found in this study. The orsellinic acid, formed from acetyl-CoA and malonyl-CoA via the polyketide pathway, was found to be a novel benzoquinone ring precursor for AQ and 4- AAQB. The identification of endogenously synthesized farnesylated intermediates allows us to postulate the routes of AQ and 4- AAQB biosynthesis in A. cinnamomea. ? 2016 American Chemical Society.
|Appears in Collections:||食品科技研究所|
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