Enhancement of 4-acetylantroquinonol B production by supplementation of its precursor during submerged fermentation of antrodia cinnamomea
Journal
Journal of Agricultural and Food Chemistry
Journal Volume
61
Journal Issue
38
Pages
9160-9165
Date Issued
2013
Author(s)
Abstract
The antiproliferation activity of the ethanol extract of A. cinnamomea mycelium on hepatocellular cancer cells HepG2 was found to be associated with aroma intensity of the broth during fermentation. We hypothesized that some of the volatile compounds are the precursors of the key bioactive component 4-acetylantroquinonol B of this fungus. The major volatile compounds of A. cinnamomea were identified by GC/MS, and they are oct-1-en-3-ol, linalool, methyl phenylacetate, nerolidol, £^-cadinene and 2,4,5- trimethoxybenzaldehyde (TMBA). TMBA and nerolidol were further selected and used as supplements during fermentation. It was found that both of them could increase the production of 4-acetylantroquinonol B and enhance the antiproliferation activity of the fungus. In addition, the TMBA was identified as the most promising supplement for increasing the bioactivity of A. cinnamomea during cultivation. ? 2013 American Chemical Society.
Subjects
2,4,5-trimethoxybenzaldehyde
4-acetylantroquinonol B
Antrodia cinnamomea
GC/MS
nerolidol
submerged fermentation
volatile compounds
SDGs
Other Subjects
2,4,5-trimethoxybenzaldehyde; Antrodia cinnamomea; GC/MS; Nerolidol; Submerged fermentation; Volatile compounds; Fermentation; Volatile organic compounds; 4 acetylantroquinonol B; 4-acetylantroquinonol B; antineoplastic agent; cyclohexanone derivative; drug derivative; gamma butyrolactone; Antrodia; article; biosynthesis; cell proliferation; chemistry; culture medium; drug effect; fermentation; HepG2 cell line; human; mass fragmentography; metabolism; 4-Butyrolactone; Antineoplastic Agents; Antrodia; Cell Proliferation; Culture Media; Cyclohexanones; Fermentation; Gas Chromatography-Mass Spectrometry; Hep G2 Cells; Humans
Type
journal article