https://scholars.lib.ntu.edu.tw/handle/123456789/413340
Title: | A Modified Procedure for Caseinophosphopeptide Analysis | Authors: | Lin Jr. H. Chiang B.-H. |
Issue Date: | 1996 | Journal Volume: | 34 | Journal Issue: | 8 | Start page/Pages: | 358-361 | Source: | Journal of Chromatographic Science | Abstract: | A modified procedure is established for analyzing caseinophosphopeptides. The sodium casemate hydrolysate is first treated by immobilized metal ion affinity chromatography to enrich the phosphopeptides. Because of the formation of Fe3+-peptide complexes, ethylenediaminetetraacetic acid is added to the bound fraction eluted with the immobilized metal ion affinity chromatography to disintegrate the complexes. Thus, the subsequent high-performance liquid Chromatographic analysis is facilitated. A stepwise gradient elution is also suggested to enhance the resolution of caseinophosphopeptides during high-performance liquid chromatographic analysis. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/413340 | ISSN: | 00219665 | DOI: | 10.1093/chromsci/34.8.358 https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030215639&doi=10.1093%2fchromsci%2f34.8.358&partnerID=40&md5=6c4f8e951ed2e8f06fae5beae6f1e825 |
Appears in Collections: | 食品科技研究所 |
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34-8-358.pdf | 772.24 kB | Adobe PDF | View/Open |
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