https://scholars.lib.ntu.edu.tw/handle/123456789/413369
標題: | Glycyrrhetinic acid suppressed NF-£eB activation in TNF-£\-induced hepatocytes | 作者: | Chen H.-J. Kang S.-P. Lee I.-J. Lin Y.-L. |
關鍵字: | glycyrrhetinic acid;Glycyrrhiza uralensis;hepatocytes;NF-£eB;TNF-£\ | 公開日期: | 2014 | 卷: | 62 | 期: | 3 | 起(迄)頁: | 618-625 | 來源出版物: | Journal of Agricultural and Food Chemistry | 摘要: | Tumor necrosis factor-alpha (TNF-£\) is a crucial inflammatory cytokine when hepatocytes are damaged. Glycyrrhiza uralensis Fisch. (Chinese licorice) has been widely used in Chinese herbal prescriptions for the treatment of liver diseases and as a food additive. Nuclear factor-kappa B (NF-£eB) reporter gene assay in TNF-£\-induced HepG2 was used as a screening platform. I£eB£\ phosphorylation and p65 translocation were measured by Western blotting, and nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) gene expression were further confirmed in rat primary hepatocytes. Results showed that TNF-£\ enhanced NF-£eB activity was significantly attenuated by glycyrrhetinic acid in a concentration-dependent manner in the NF-£eB reporter gene assay. Glycyrrhetinic acid decreased the gene expression of iNOS through inhibited I£eB£\ phosphorylation and p65 translocation in protein level. Furthermore, NO production and iNOS expression were reduced by glycyrrhetinic acid in TNF-£\-induced rat primary hepatocytes. These results suggest that glycyrrhetinic acid may provide hepatoprotection against chronic liver inflammation through attenuating NF-£eB activation to alleviate the inflammation. ? 2014 American Chemical Society. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/413369 | ISSN: | 00218561 | DOI: | 10.1021/jf405352g | SDG/關鍵字: | Concentration-dependent manners; Glycyrrhetinic acids; Glycyrrhiza uralensis; Glycyrrhiza uralensis Fisch; Hepatocytes; Inducible nitric oxide synthase (iNOS); Nuclear factor kappaB; Tumor necrosis factor-alpha; Concentration-dependent manners; Glycyrrhetinic acids; Glycyrrhiza uralensis; Glycyrrhiza uralensis Fisch; Hepatocytes; Inducible nitric oxide synthase (iNOS); Nuclear factor kappaB; Tumor necrosis factor alpha; Gene expression; Nitric oxide; Pathology; Phosphorylation; Rats; Chemical activation; Diagnosis; Genes; Nitric oxide; Pathology; Phosphorylation; Rats; Diagnosis; Gene expression; Glycyrrhiza uralensis; Rattus; glycyrrhetinic acid; herbaceous agent; I kappa B; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; tumor necrosis factor alpha; animal; chemistry; down regulation; drug effects; Glycyrrhiza uralensis; human; liver cell; metabolism; phosphorylation; rat; signal transduction; Sprague Dawley rat; Animals; Down-Regulation; Drugs, Chinese Herbal; Glycyrrhetinic Acid; Glycyrrhiza uralensis; Hepatocytes; Humans; I-kappa B Proteins; NF-kappa B; Nitric Oxide Synthase Type II; Phosphorylation; Rats; Rats, Sprague-Dawley; Signal Transduction; Tumor Necrosis Factor-alpha |
顯示於: | 食品科技研究所 |
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