https://scholars.lib.ntu.edu.tw/handle/123456789/413401
標題: | Enzymatic hydrolysates obtained from Trametes versicolor polysaccharopeptides protect human skin keratinocyte against AAPH?induced oxidative stress and inflammatory | 作者: | Chou C.-H. Tsai M.-S. Lu H.-Y. Chang C.-K. Cheng K.-C. Jhan M.-H. KUAN-CHEN CHENG |
關鍵字: | antioxidant and anti-inflammatory properties;enzymatic hydrolysates;NF-£eB signaling pathway;polysaccharopeptides;Trametes versicolor | 公開日期: | 2019 | 來源出版物: | Journal of Cosmetic Dermatology | 摘要: | Background: Polysaccharopeptides (PSPs) extracted from Trametes versicolor show antitumor, anti-inflammatory, and immunomodulation effects. According to our previous report, the enzymatic hydrolysates obtained from T?versicolor PSPs by 80?U/mL £]-1,3-D-glucanase (PSPs-EH80) did not change the functional groups of PSPs but enhanced their antioxidative activities. However, the mechanism elevating the antioxidant and anti-inflammatory effect of PSPs-EH80 is not clear. Aims: This research focused on the protective mechanism(s) of PSPs-EH80 against free radical and 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced oxidative damage in human keratinocyte (HaCaT) cells. Methods: We evaluated the anti-inflammatory potential of PSPs-EH80 by assessing its free radical-induced oxidative damage. Using the HaCaT cell as the experimental system, we tested the protective effects of PSPs-EH80 on a model of AAPH-induced cellular oxidative damage through the assessment of cell survival rate. Heme oxygenase 1 (HO-1), nuclear factor erythroid 2-related factor 2 (Nrf2), cyclooxygenase-2 (COX-2), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-£eB), extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase were determined using MTT assays and Western blotting. Results: We demonstrated that PSPs-EH80 significantly enhanced keratinocyte viability, and augmented the antioxidant HO-1 expressions through upregulation of the Nrf2, compared with PSPs. Furthermore, PSPs-EH80 significantly reduced AAPH-induced COX-2 expressions through downregulation of the ERK, p38, and NF-£eB signaling pathways. Conclusion: The PSPs-EH80 exhibits a stronger antioxidant and anti-inflammatory capacity than PSPs. Therefore, PSPs-EH80 could be effective for attenuating free radical-induced oxidative damage in human skin and can be applied widely in the fields of cosmetics and medicine. ? 2019 Wiley Periodicals, Inc. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/413401 | ISSN: | 14732130 | DOI: | 10.1111/jocd.12959 | SDG/關鍵字: | 2,2' azobis(2 amidinopropane); antiinflammatory agent; heme oxygenase 1; immunoglobulin enhancer binding protein; mitogen activated protein kinase; plant extract; polysaccharopeptide; stress activated protein kinase; Trametes versicolor extract; transcription factor Nrf2; transcription factor RelA; unclassified drug; antioxidant; polysaccharide peptide; protein hydrolysate; proteoglycan; antiinflammatory activity; antioxidant activity; Article; cell damage; cell protection; cell survival; controlled study; down regulation; electrophoresis; enzymatic hydrolysis; enzyme activation; HaCat cell line; human; human cell; inflammation; keratinocyte; MTT assay; nonhuman; oxidation reduction state; oxidative stress; priority journal; protein expression; Trametes versicolor; upregulation; Western blotting; cell culture; chemistry; cytology; drug effect; enzymology; inflammation; isolation and purification; keratinocyte; oxidative stress; skin; Trametes; Antioxidants; Cells, Cultured; Humans; Inflammation; Keratinocytes; Oxidative Stress; Protein Hydrolysates; Proteoglycans; Skin; Trametes |
顯示於: | 生物科技研究所 |
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