https://scholars.lib.ntu.edu.tw/handle/123456789/413458
標題: | Evaluation of medium composition and fermentation parameters on pullulan production by Aureobasidium pullulans | 作者: | Cheng K.-C. Demirci A. KUAN-CHEN CHENG |
關鍵字: | Aureobasidium pullulans;Exopolysaccharide;Fermentation;FTIR;Pullulan | 公開日期: | 2011 | 卷: | 17 | 期: | 2 | 起(迄)頁: | 99-109 | 來源出版物: | Food Science and Technology International | 摘要: | The goal of this study was to enhance pullulan production by evaluating the effects of different fermentation parameters. Various carbon sources and their concentrations, yeast extract (YE) concentrations, fermentation temperatures and various pH profiles were examined. The optimal growth condition for pullulan production by Aureobasidium pullulans has been found as 75 g/L of sucrose as carbon source, 3 g/L of YE and cultivation temperature at 30 ¢XC. Under these conditions with an initial pH at 5, 20.7 g/L of final pullulan concentration and 0.22 g/L/h maximum production rate were obtained. Later on, various pH profiles, agitation speeds, aerations and fed-batch fermentation were evaluated. The results demonstrated that pullulan production was enhanced to 25.8 g/L after 7-day cultivation with a 0.68 -g/L/h maximum production rate. There was no significant improvement of pullulan production from fed-batch fermentation. The optimal kinetics parameters were as follows: initial pH at 2.0, switched to pH 5.0 after 72 h and kept constant; agitation speed at 200 rpm; aeration at 1.5 vvm. The quality analysis demonstrated that the pullulan content produced from optimal conditions was 94.5% and its viscosity was 2.3 centipoise (cP). Fourier transform infrared spectroscopy also suggested that pullulan dominated the produced exopolysaccharide. ? SAGE Publications 2011. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/413458 | ISSN: | 10820132 | DOI: | 10.1177/1082013210368719 |
顯示於: | 生物科技研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。