|Title:||Antcin K, an active triterpenoid from the fruiting bodies of basswood-cultivated antrodia cinnamomea, inhibits metastasis via suppression of integrin-mediated adhesion, migration, and invasion in human hepatoma cells||Authors:||Huang Y.-L.
|Keywords:||antcin K;Antrodia cinnamomea;epithelial mesenchymal transition (EMT);liver cancer metastasis;matrix metalloproteinases (MMPs)||Issue Date:||2015||Journal Volume:||63||Journal Issue:||18||Start page/Pages:||4561-4569||Source:||Journal of Agricultural and Food Chemistry||Abstract:||
Previous research demonstrated that the ethyl acetate extract from Antrodia cinnamomea suppresses the invasive potential of human breast and hepatoma cells, but the effective compounds are not identified. The main bioactive compounds of A. cinnamomea are ergostane-type triterpenoids, and the content of antcin K is the highest. The objective of this study was to evaluate the antimetastatic activity and mechanisms of antcin K purified from the fruiting body of basswood-cultivated A. cinnamomea on human liver cancer Hep 3B cells. The results showed that adhesion, migration, and invasion of Hep 3B cells were effectively inhibited by antcin K within 24 h of treatment. Antcin K not only reduced the protein expression and activity of MMP-2 and MMP-9 but also down-regulated vimentin and up-regulated E-cadherin in Hep 3B cells. In depth investigation for the molecular mechanism revealed that antcin K could reduce the protein expression of integrin £]1, £]3, £\5, and £\v and suppress phosphorylation of FAK, Src, PI3K, AKT, MEK, ERK, and JNK. These results suggested that antcin K was able to inhibit the metastasis of human hepatoma cells through suppression of integrin-mediated adhesion, migration, and invasion. Coupled with these findings, antcin K has a good potential to reduce the risk of liver cancer metastasis. ? 2015 American Chemical Society.
Adhesion; Cells; Diseases; Pathology; Proteins; Tumors; Antrodia cinnamomea; Bioactive compounds; Epithelial-mesenchymal transition; Human hepatoma cells; Liver cancers; Matrix metalloproteinases; Molecular mechanism; Protein expressions; Cytology; Antrodia cinnamomea; Malvaceae; antcin K; antineoplastic agent; cholestane derivative; integrin; Antrodia; Carcinoma, Hepatocellular; cell adhesion; cell motion; chemistry; drug effects; fruiting body; genetics; growth, development and aging; human; isolation and purification; Liver Neoplasms; metabolism; metastasis; pathophysiology; Tilia; tumor cell line; tumor invasion; Antineoplastic Agents, Phytogenic; Antrodia; Carcinoma, Hepatocellular; Cell Adhesion; Cell Line, Tumor; Cell Movement; Cholestenes; Fruiting Bodies, Fungal; Humans; Integrins; Liver Neoplasms; Neoplasm Invasiveness; Neoplasm Metastasis; Tilia
|Appears in Collections:||食品科技研究所|
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