https://scholars.lib.ntu.edu.tw/handle/123456789/413579
標題: | Protective effects of water extract of clam on normal and CCl 4-induced damage in primary cultured rat hepatocytes | 作者: | Chi H.M. Chou S.T. Lin S.C. Su Z.Y. Sheen L.-Y. |
關鍵字: | Carbon Tetrachloride;Clam;Glutathione;Glutathione- Related Enzymes;Primary Rat Hepatocytes | 公開日期: | 2010 | 卷: | 38 | 期: | 6 | 起(迄)頁: | 1193-1205 | 來源出版物: | American Journal of Chinese Medicine | 摘要: | The objective of this study was to investigate the effects of various concentrations and incubation times of water extract of clam (WEC) on glutathione, its antioxidant and the detoxification defense systems in normal and CCl4-induced oxidative damaged primary rat hepatocytes. This study showed that when the hepatocytes were treated with WEC(0.14 ? 1.68 mg/ml), the intracellular glutathione (GSH) levels, GSH/GSSG ratio, and the activities of GSH-related enzymes (GPx, GRd, and GST) were higher than those in the control at 24 or 48 hour treatments. However, the lactate dehydrogenase (LDH) leakage and microscopic observations did not differ from those of the control. Yet, when the hepatocytes were pretreated with various concentrations of WEC for 24 hours and then exposed to 5 mM carbon tetrachloride (CCl 4) for 1 hour, at concentrations of WEC between 0.42 ? 1.68 mg/ml, the viabilities, intracellular GSH level, and activities of GST and GPx were significantly increased compared to those of the CCl4-treated control group (p < 0.05). In conclusion, WEC could improve the viability and the capabilities of detoxification and antioxidation in hepatocytes by increasing the GSH level and the activities of GSH-related enzymes. ? 2010 World Scientific Publishing Company & Institute for Advanced Research in Asian Science and Medicine. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/413579 | ISSN: | 0192415X | DOI: | 10.1142/S0192415X10008561 |
顯示於: | 食品科技研究所 |
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