Antioxidative protection of water extra of psoralea fruit and bakuchiol on primary rat hepatocytes

Abstract

Protective effect of water extract of psoralea fruit and bakuchiol on the viability, lipid peroxidation, glutathione (GSH) levels and activities of GSH-related enzymes of primary rat hepatocytes under oxidative stress induced by glucose/glucose oxidase was investigated. Hepatocytes were incubated with or without water extract of psoralea fruit (0.6 mg/mL) or bakuchiol (0.125 mM), and 50 £gg/mL glucose, 100 £gg/mL glucose oxidase except for the blank. The results reveal that the lactate dehydrogenase leakage of hepatocytes incubated with water extract of psoralea fruit or bakuchiol was significantly suppressed; showing only about half that of the control at 8 hr incubation, and the protective effects lasted till 48 hr (p < 0.05). As for the lipid peroxidation of hepatocytes, water extract of psoralea fruit or bakuchiol significantly (p < 0.05) controlled the lipid peroxidation induced by glucose and glucose oxidase. On the other hand, water extract of psoralea fruit or bakuchiol helped maintain GSH and oxidized glutathione levels in hepatocytes under oxidative stress, and even induced more GSH production. Regarding the aspect of the GSH-related enzymes activities, water extract of psoralea fruit or bakuchiol stopped the activities of glutathione peroxidase, glutathione S- transferase and glutathione reductase decrease what caused by oxidative stress and even to elevate the activities of them. Thus, water extract of psoralea fruit and bakuchiol protected hepatocytes from oxidation damage via increasing the GSH levels and elevating the activities of GSH-related enzymes of hepatocytes.