https://scholars.lib.ntu.edu.tw/handle/123456789/413663| Title: | Viable but non-culturable state and toxin gene expression of enterohemorrhagic Escherichia coli O157 under cryopreservation | Authors: | Liu J. Zhou R. Li L. Peters B.M. Li B. Lin C.-W. Chuang T.-L. Chen D. Zhao X. Xiong Z. Xu Z. Shirtliff M.E. |
Keywords: | Cryopreservation; E. coli O157; PMA-PCR; Toxin expression; VBNC state | Issue Date: | 2017 | Publisher: | Elsevier Masson SAS | Journal Volume: | 168 | Journal Issue: | 3 | Start page/Pages: | 188-193 | Source: | Research in Microbiology | Abstract: | As major food-borne pathogens worldwide, Escherichia coli are capable of toxin production directly causing severe human disease. However, routine methods are incapable of detecting viable but non-culturable (VBNC) bacteria in food products and raw materials, leading to false-negative identification. In this study, VBNC E. coli O157 strains were acquired after cryopreservation at ?20?°C, with and without freeze-thawing; morphology was observed to be of shorter rod-shape, and toxin expression remained at relatively high levels. PMA-PCR assay for VBNC detection was also validated. Therefore, these results suggest that VBNC E. coli O157 strains may represent a strong threat to public health and food safety. ? 2016 Institut Pasteur |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85007425406&doi=10.1016%2fj.resmic.2016.11.002&partnerID=40&md5=60e46e425731732dd03894fc6db8403b https://scholars.lib.ntu.edu.tw/handle/123456789/413663 |
ISSN: | 9232508 | DOI: | 10.1016/j.resmic.2016.11.002 | SDG/Keyword: | Shiga toxin; bacterial toxin; Article; bacterial strain; controlled study; cryopreservation; enterohemorrhagic Escherichia coli; gene expression; nonhuman; polymerase chain reaction; priority journal; cytology; Escherichia coli O157; food safety; genetics; human; isolation and purification; microbial viability; physiology; scanning electron microscopy; Bacterial Toxins; Cryopreservation; Escherichia coli O157; Food Safety; Humans; Microbial Viability; Microscopy, Electron, Scanning; Polymerase Chain Reaction [SDGs]SDG3 |
| Appears in Collections: | 醫學工程學研究所 |
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