|Title:||Viable but non-culturable state and toxin gene expression of enterohemorrhagic Escherichia coli O157 under cryopreservation||Authors:||Liu J.
|Keywords:||Cryopreservation; E. coli O157; PMA-PCR; Toxin expression; VBNC state||Issue Date:||2017||Publisher:||Elsevier Masson SAS||Journal Volume:||168||Journal Issue:||3||Start page/Pages:||188-193||Source:||Research in Microbiology||Abstract:||
As major food-borne pathogens worldwide, Escherichia coli are capable of toxin production directly causing severe human disease. However, routine methods are incapable of detecting viable but non-culturable (VBNC) bacteria in food products and raw materials, leading to false-negative identification. In this study, VBNC E. coli O157 strains were acquired after cryopreservation at ?20?°C, with and without freeze-thawing; morphology was observed to be of shorter rod-shape, and toxin expression remained at relatively high levels. PMA-PCR assay for VBNC detection was also validated. Therefore, these results suggest that VBNC E. coli O157 strains may represent a strong threat to public health and food safety. ? 2016 Institut Pasteur
|ISSN:||9232508||DOI:||10.1016/j.resmic.2016.11.002||SDG/Keyword:||Shiga toxin; bacterial toxin; Article; bacterial strain; controlled study; cryopreservation; enterohemorrhagic Escherichia coli; gene expression; nonhuman; polymerase chain reaction; priority journal; cytology; Escherichia coli O157; food safety; genetics; human; isolation and purification; microbial viability; physiology; scanning electron microscopy; Bacterial Toxins; Cryopreservation; Escherichia coli O157; Food Safety; Humans; Microbial Viability; Microscopy, Electron, Scanning; Polymerase Chain Reaction
|Appears in Collections:||醫學工程學研究所|
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