Interactions between bacterial surface and nanoparticles govern the performance of "chemical nose" biosensors
Journal
Biosensors and Bioelectronics
Journal Volume
83
Pages
115-125
Date Issued
2016
Author(s)
Abstract
Rapid and portable diagnosis of pathogenic bacteria can save lives lost from infectious diseases. Biosensors based on a "chemical nose" approach are attracting interest because they are versatile but the governing interactions between bacteria and the biosensors are poorly understood. Here, we use a "chemical nose" biosensor based on gold nanoparticles to explore the role of extracellular polymeric substances in bacteria-nanoparticle interactions. We employ simulations using Maxwell-Garnett theory to show how the type and extent of aggregation of nanoparticles influence their colorimetric response to bacteria. Using eight different species of Gram-positive and Gram-negative bacteria, we demonstrate that this "chemical nose" can detect and identify bacteria over two orders of magnitude of concentration (89% accuracy). Additionally, the "chemical nose" differentiates between binary and tertiary mixtures of the three most common hospital-isolated pathogens: Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa (100% accuracy). We demonstrate that the complex interactions between nanoparticles and bacterial surface determine the colorimetric response of gold nanoparticles and thus, govern the performance of "chemical nose" biosensors. ? 2016 Elsevier B.V.
Subjects
Colorimetric; Extracellular polymeric substances; Gold nanoparticles; Lipid blots; Modeling; Pathogens
SDGs
Other Subjects
Binary mixtures; Biosensors; Color; Colorimetry; Diagnosis; Escherichia coli; Fiber optic sensors; Gold; Gold compounds; Metal nanoparticles; Models; Nanoparticles; Pathogens; Polymers; Colorimetric; Extra-cellular polymeric substances; Gold Nanoparticles; Gram-negative bacteria; Maxwell-garnett theory; Nanoparticle interaction; Pseudomonas aeruginosa; Staphylococcus aureus; Bacteria; gold nanoparticle; gold; metal nanoparticle; polymer; Article; bacteriology; bacterium culture; binding affinity; biosensor; centrifugation; chemical nose biosensor; colony forming unit; colorimetry; Escherichia coli; Gram negative bacterium; Gram positive bacterium; hydrophobicity; limit of detection; nonhuman; principal component analysis; protein binding; Pseudomonas aeruginosa; simulation; Staphylococcus aureus; transmission electron microscopy; bacterium; chemistry; cytology; genetic procedures; procedures; ultrastructure; Bacteria; Biosensing Techniques; Colorimetry; Gold; Metal Nanoparticles; Polymers
Publisher
Elsevier Ltd
Type
journal article