Optimal hepatitis B virus primer sequence design for isothermal amplification
Journal
Biomedical Engineering - Applications, Basis and Communications
Journal Volume
19
Journal Issue
3
Pages
137-144
Date Issued
2007
Author(s)
Abstract
We have reported the successful use of the Loop Mediated Isothermal Amplification (LAMP) reaction for Hepatitis B Virus (HBV) DNA in a 25 μl micro-reactor for the development of a rapid diagnostic device recently. This method shows the successful amplification of HBV DNA with high specificity and sensitivity efficiently within one hour. Such a powerful method can be used for many other fast diagnostic applications with the help of optimal primer design and miniaturization of reactor. In this report, we will first summarize the general design rules for primer design from literature reviews and our experimental results. We will discuss the effects of HBV primers and concentrations of working buffers for optimal performance. ? 2007 National Taiwan University.
SDGs
Other Subjects
Electric lamps; Isotherms; Diagnostic applications; Hepatitis B virus; Isothermal; Isothermal amplifications; Literature reviews; Loop mediated isothermal amplifications; Optimal performance; Primer design; Viruses; betaine; buffer; virus DNA; article; concentration response; crystallization; diagnostic value; DNA sequence; early diagnosis; electronics; gene amplification; gene sequence; Hepatitis B virus; loop mediated isothermal amplification; mathematical computing; melting point; nonhuman; reactor design; sensitivity and specificity; Hepatitis B virus
Publisher
World Scientific Publishing Co. Pte Ltd
Type
journal article