Chinese olive (Canarium album l.) fruit extract attenuates metabolic dysfunction in diabetic rats
Journal
Nutrients
Journal Volume
9
Journal Issue
10
Date Issued
2017-10-15
Author(s)
Abstract
© 2017 by the authors. Licensee MDPI, Basel, Switzerland. Hyperglycemia and dysregulation of lipid metabolism play a crucial role in metabolic dysfunction. The aims of present study were to evaluate the ameliorative effect of the ethyl acetate fraction of Chinese olive fruit extract (CO-EtOAc) on high-fat diet (HFD) and streptozotocin (STZ)-induced diabetic rats. CO-EtOAc, rich in gallic acid and ellagic acid, could markedly decreased the body weight and epididymal adipose mass. In addition, CO-EtOAc increased serum HDL-C levels, hepatic GSH levels, and antioxidant enzyme activities; lowered blood glucose, serum levels of total cholesterol (TC), triglycerides (TG), bile acid, and tumor necrosis factor alpha (TNFα); and reduced TC and TG in liver. We further demonstrated that CO-EtOAc mildly suppressed hepatic levels of phosphorylated IRS-1, TNF-α, and IL-6, but enhanced Akt phosphorylation. The possible mechanisms of cholesterol metabolism were assessed by determining the expression of genes involved in cholesterol transportation, biosynthesis, and degradation. It was found that CO-EtOAc not only inhibited mRNA levels of SREBP-2, HMG-CoAR, SR-B1, and CYP7A1 but also increased the expression of genes, such as ABCA1 and LDLR that governed cholesterol efflux and cholesterol uptake. Moreover, the protein expressions of ABCA1 and LDLR were also significantly increased in the liver of rats supplemented with CO-EtOAc. We suggest that Chinese olive fruit may ameliorate metabolic dysfunction in diabetic rats under HFD challenge.
Subjects
Antioxidant activities | Chinese olive fruit | High-fat diet | Hyperglycemia | Metabolic dysfunction | Proinflammatory cytokines
Antioxidant activities; Chinese olive fruit; High-fat diet; Hyperglycemia; Metabolic dysfunction; Proinflammatory cytokines
SDGs
Other Subjects
ABC transporter A1; antilipemic agent; bile acid; Canarium album extract; catalase; cholesterol; cholesterol 7alpha monooxygenase; ellagic acid; gallic acid; glucose; glutathione; glutathione disulfide; glutathione peroxidase; high density lipoprotein cholesterol; hydroxymethylglutaryl coenzyme A reductase; insulin receptor substrate 1; interleukin 6; low density lipoprotein receptor; plant extract; protein kinase B; scavenger receptor BI; sterol regulatory element binding protein 2; superoxide dismutase; thiobarbituric acid reactive substance; triacylglycerol; tumor necrosis factor; unclassified drug; antidiabetic agent; antioxidant; insulin; plant extract; thiobarbituric acid reactive substance; animal experiment; animal model; animal tissue; Article; Burseraceae; Canarium album; cholesterol degradation; cholesterol metabolism; cholesterol synthesis; cholesterol transport; controlled study; enzyme activity; experimental diabetes mellitus; food intake; gene expression; high performance liquid chromatography; histopathology; insulin signaling; lipid diet; liver tissue; male; metabolic disorder; nonhuman; protein expression; protein phosphorylation; rat; real time polymerase chain reaction; reverse transcription polymerase chain reaction; weight reduction; Western blotting; animal; body weight; Burseraceae; chemistry; drug effect; experimental diabetes mellitus; fruit; gene expression regulation; liver; metabolism; signal transduction; Sprague Dawley rat; Animals; Antioxidants; Body Weight; Burseraceae; Chromatography, High Pressure Liquid; Diabetes Mellitus, Experimental; Fruit; Gene Expression Regulation, Enzymologic; Hypoglycemic Agents; Insulin; Liver; Male; Plant Extracts; Rats; Rats, Sprague-Dawley; Signal Transduction; Thiobarbituric Acid Reactive Substances
Publisher
MDPI
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
nutrients-09-01123.pdf
Size
2.86 MB
Format
Adobe PDF
Checksum
(MD5):52fe127ed789fac5e071b235d0175292