https://scholars.lib.ntu.edu.tw/handle/123456789/414024
標題: | Characteristics of fucose-containing polysaccharides from submerged fermentation of Agaricus blazei Murill | 作者: | Wang H.-T. Yang L.-C. Yu H.-C. Chen M.-L. Wang H.-J. HUEI WANG |
關鍵字: | Acidic polysaccharide;Agaricus blazei Murill;Fucose;Galactan;TNF-£\ stimulation | 公開日期: | 2018 | 卷: | 26 | 期: | 2 | 起(迄)頁: | 678-687 | 來源出版物: | Journal of Food and Drug Analysis | 摘要: | Fucose is one of important residues of recognition pattern for many immune cells. In this study, we characterized bioactive fucose-containing acidic polysaccharides from submerged fermentation of Agaricus blazei Murill. We obtained the polysaccharides through a cell-based activity-guided strategy, and used carbohydrate recognition monoclonal antibodies based Enzyme-Linked Immuno Sorbent Assay (ELISA) along with methylation and NMR analyses to investigate the structural characteristics of the polysaccharides. The polysaccharides had Mw of 3.5 ¡Ñ 10 5 Da. The major sugars were L-fucose, L-arabinose, D-galactose, D-xylose, and D-galacturonic acid in the molar ratio of 6.4, 15.5, 28.5, 14.7, and 25.0% with a small amount of D-glucose, D-mannose, L-rhamnose, and D-glucuronic acid. Results indicated that the bioactive polysaccharides consisted of a (1,4)-Galp and (1,4)-GalAp back bone; (1,2)-Xyl and (1,2)-Rha might also comprise backbone or constitute side chain; linkage (1,5)-Ara and terminal fucosyl residues were also involved in the polysaccharides. Regarding bioactivity, removal of the terminal L-fucosyl residues reduced the TNF-£\ cytokine stimulating activity of the polysaccharides in a RAW 264.7 macrophage cell-line test, whereas NF-£eB and TLR4 affected the polysaccharide-induced TNF-£\ production. ? 2017 |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/414024 | ISSN: | 10219498 | DOI: | 10.1016/j.jfda.2017.07.006 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028672022&doi=10.1016%2fj.jfda.2017.07.006&partnerID=40&md5=b03f1594ab3b9ccc4277931d962e7eb5 |
顯示於: | 食品科技研究所 |
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