https://scholars.lib.ntu.edu.tw/handle/123456789/414095
標題: | Cellular uptake and cytotoxicity of chitosan-caseinophosphopeptides nanocomplexes loaded with epigallocatechin gallate | 作者: | Hu B. Ting Y. Zeng X. Huang Q. |
關鍵字: | Cellular toxicity;Cellular uptake;Chitosan-caseinophosphopeptides nanoparticles;Epigallocatechin gallate;Intestinal absorption | 公開日期: | 2012 | 卷: | 89 | 期: | 2 | 起(迄)頁: | 362-370 | 來源出版物: | Carbohydrate Polymers | 摘要: | Epigallocatechin gallate (EGCG) was successfully encapsulated in novel nanocomplexes assembled from bioactive peptides, caseinophosphopeptides (CPPs), and chitosan (CS), a natural cationic polymer. Their particle sizes and surface charges were determined to be in the range of 150.0 ¡Ó 4.3 nm and 32.2 ¡Ó 3.3 mV respectively. Crosslinking between the NH 3 + groups of CS with the PO - and COO - groups of CPP, as well as the hydrogen bonding were confirmed from the FTIR results. Atomic force microscopy (AFM) images showed that EGCG loaded CS-CPP nanocomplexes were spherical in shape. Maintaining the surface charge as high as +32.2 mV, crosslinking CS with peptides reduced the cytotoxicity of CS nanoparticles. In addition, cellular internalization of EGCG-loaded CS-CPP nanoparticles was confirmed from green fluorescence inside the Caco-2 cells. The process of nanoparticle uptake was dose and time dependent in the range of time and concentration studied. Furthermore, the intestinal permeability of EGCG using Caco-2 monolayer was enhanced significantly as delivered by nanoparticles, which indicated the promising elevation of EGCG bioavailability. ? 2012 Elsevier Ltd. All rights reserved. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/414095 | ISSN: | 01448617 | DOI: | 10.1016/j.carbpol.2012.03.015 |
顯示於: | 食品科技研究所 |
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