https://scholars.lib.ntu.edu.tw/handle/123456789/414102
標題: | CHAPERONIN 20 mediates iron superoxide dismutase (FeSOD) activity independent of its co-chaperonin role in Arabidopsis chloroplasts | 作者: | Kuo, W. Y. Huang, C. H. Liu, A. C. CHIU-PING CHENG SHU-HSING LI Chang, W. C. Weiss, C. Azem, A. TSUNG-LUO JINN |
關鍵字: | Chaperonin system | Chloroplast development | Co-chaperonin | FeSOD | Oxidative stress | ROS signaling | SOD | 公開日期: | 1-一月-2013 | 出版社: | WILEY | 卷: | 197 | 期: | 1 | 起(迄)頁: | 99 | 來源出版物: | New Phytologist | 摘要: | Iron superoxide dismutases (FeSODs; FSDs) are primary antioxidant enzymes in Arabidopsis thaliana chloroplasts. The stromal FSD1 conferred the only detectable FeSOD activity, whereas the thylakoid membrane- and nucleoid-co-localized FSD2 and FSD3 double mutant showed arrested chloroplast development. FeSOD requires cofactor Fe for its activity, but its mechanism of activation is unclear. We used reversed-phase high-performance liquid chromatography (HPLC), gel filtration chromatography, LC-MS/MS, protoplast transient expression and virus-induced gene silencing (VIGS) analyses to identify and characterize a factor involved in FeSOD activation. We identified the chloroplast-localized co-chaperonin CHAPERONIN 20 (CPN20) as a mediator of FeSOD activation by direct interaction. The relationship between CPN20 and FeSOD was confirmed by in vitro experiments showing that CPN20 alone could enhance FSD1, FSD2 and FSD3 activity. The in vivo results showed that CPN20-overexpressing mutants and mutants with defective co-chaperonin activity increased FSD1 activity, without changing the chaperonin CPN60 protein level, and VIGS-induced downregulation of CPN20 also led to decreased FeSOD activity. Our findings reveal that CPN20 can mediate FeSOD activation in chloroplasts, a role independent of its known function in the chaperonin system. © 2012 New Phytologist Trust. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/414102 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84870249599&doi=10.1111%2fj.1469-8137.2012.04369.x&partnerID=40&md5=1610dd34f6fb24fd552e5af81a53b0e3 |
ISSN: | 0028646X | DOI: | https://api.elsevier.com/content/abstract/scopus_id/84870249599 10.1111/j.1469-8137.2012.04369.x |
顯示於: | 植物科學研究所 |
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j.1469-8137.2012.04369.x (1).pdf | 1.64 MB | Adobe PDF | 檢視/開啟 |
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