https://scholars.lib.ntu.edu.tw/handle/123456789/414108
標題: | Transient production of artemisinin in Nicotiana benthamiana is boosted by a specific lipid transfer protein from A. annua | 作者: | Wang, Bo Kashkooli, Arman Beyraghdar Sallets, Adrienne HIENG MING TING de Ruijter, Norbert C.A. Olofsson, Linda Brodelius, Peter Pottier, Mathieu Boutry, Marc Bouwmeester, Harro van der Krol, Alexander R. |
關鍵字: | ABC transporters | Artemisia annua | Artemisinin | Lipid transfer proteins | Nicotiana benthamiana | Pleiotropic Drug Resistance protein | 公開日期: | 1-十一月-2016 | 出版社: | ACADEMIC PRESS INC ELSEVIER SCIENCE | 卷: | 38 | 起(迄)頁: | 159 | 來源出版物: | Metabolic Engineering | 摘要: | © 2016 International Metabolic Engineering Society Our lack of full understanding of transport and sequestration of the heterologous products currently limit metabolic engineering in plants for the production of high value terpenes. For instance, although all genes of the artemisinin/arteannuin B (AN/AB) biosynthesis pathway (AN-PW) from Artemisia annua have been identified, ectopic expression of these genes in Nicotiana benthamiana yielded mostly glycosylated pathway intermediates and only very little free (dihydro)artemisinic acid [(DH)AA]. Here we demonstrate that Lipid Transfer Protein 3 (AaLTP3) and the transporter Pleiotropic Drug Resistance 2 (AaPDR2) from A. annua enhance accumulation of (DH)AA in the apoplast of N. benthamiana leaves. Analysis of apoplast and cell content and apoplast exclusion assays show that AaLTP3 and AaPDR2 prevent reflux of (DH)AA from the apoplast back into the cells and enhances overall flux through the pathway. Moreover, AaLTP3 is stabilized in the presence of AN-PW activity and co-expression of AN-PW+AaLTP3+AaPDR2 genes yielded AN and AB in necrotic N. benthamiana leaves at 13 days post-agroinfiltration. This newly discovered function of LTPs opens up new possibilities for the engineering of biosynthesis pathways of high value terpenes in heterologous expression systems. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/414108 | ISSN: | 10967176 | DOI: | https://api.elsevier.com/content/abstract/scopus_id/84982854633 10.1016/j.ymben.2016.07.004 |
顯示於: | 植物科學研究所 |
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