https://scholars.lib.ntu.edu.tw/handle/123456789/414116
Title: | Distinct role of Arabidopsis mitochondrial P-type pentatricopeptide repeat protein-modulating editing protein, PPME, in nad1 RNA editing | Authors: | Leu K.-C. Hsieh M.-H. Wang H.-J. Hsieh H.-L. Jauh G.-Y. |
Keywords: | Arabidopsis;complex I activity;editing;mitochondria;nad1;PPR | Issue Date: | 2016 | Journal Volume: | 13 | Journal Issue: | 6 | Start page/Pages: | 593-604 | Source: | RNA Biology | Abstract: | The mitochondrion is an important power generator in most eukaryotic cells. To preserve its function, many essential nuclear-encoded factors play specific roles in mitochondrial RNA metabolic processes, including RNA editing. RNA editing consists of post-transcriptional deamination, which alters specific nucleotides in transcripts to mediate gene expression. In plant cells, many pentatricopeptide repeat proteins (PPRs) participate in diverse organellar RNA metabolic processes, but only PLS-type PPRs are involved in RNA editing. Here, we report a P-type PPR protein from Arabidopsis thaliana, P-type PPR-Modulating Editing (PPME), which has a distinct role in mitochondrial nad1 RNA editing via RNA binding activity. In the homozygous ppme mutant, cytosine (C)-to-uracil (U) conversions at both the nad1-898 and 937 sites were abolished, disrupting Arg300-to-Trp300 and Pro313-to-Ser313 amino acid changes in the mitochondrial NAD1 protein. NAD1 is a critical component of mitochondrial respiration complex I; its activity is severely reduced in the homozygous ppme mutant, resulting in significantly altered growth and development. Both abolished RNA editing and defective complex I activity were completely rescued by CaMV 35S promoter- and PPME native promoter-driven PPME genomic fragments tagged with GFP in a homozygous ppme background. Our experimental results demonstrate a distinct role of a P-type PPR protein, PPME, in RNA editing in plant organelles. ? 2016, ? Kuan-Chieh Leu, Ming-Hsiun Hsieh, Huei-Jing Wang, Hsu-Liang Hsieh, and Guang-Yuh Jauh. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/414116 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84973169057&doi=10.1080%2f15476286.2016.1184384&partnerID=40&md5=d31c65ab8e32f7d714fbab7ee33f077d |
ISSN: | 15476286 | DOI: | 10.1080/15476286.2016.1184384 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84973169057&doi=10.1080%2f15476286.2016.1184384&partnerID=40&md5=d31c65ab8e32f7d714fbab7ee33f077d |
Appears in Collections: | 植物科學研究所 |
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Distinct role of Arabidopsis mitochondrial P type pentatricopeptide repeat protein modulating editing protein PPME in nad1 RNA editing.pdf | 1.43 MB | Adobe PDF | View/Open |
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