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  3. 植物科學研究所
Please use this identifier to cite or link to this item: https://scholars.lib.ntu.edu.tw/handle/123456789/414125
Title: Comparative leaf proteomic profiling of salt-treated natural variants of Imperata cylindrica
Authors: Shih Y.-J.
Chang H.-C.
Tsai M.-C.
Wu T.-Y.
Wu T.-C.
Kao P.
Kao W.-Y.
WEN-YUAN KAO 
Keywords: 2D-DIGE;C4 Plant;Cogon grass;Imperata cylindrica var;Major;Photosynthesis;Salt stress
Issue Date: 2018
Journal Volume: 63
Journal Issue: 2
Start page/Pages: 171-182
Source: Taiwania
Abstract: 
Cogon grass (Imperata cylindrica (L.) Beauv. var. major (Nees) Hubb.) is one of the top-ten weeds worldwide. It is also a C4 medicinal plant. In particular, an ecotype from Chuwei (CW) mangrove forest was found to be salt tolerant. Comparative proteomic analysis using two-dimensional (2D)-difference in gel electrophoresis coupled with liquid chromatography-mass spectrometry (LC-MS) was carried out to identify responsive leaf proteins in the CW ecotype and salt-intolerant Sarlun (SL) population following three days of 150 mM sodium chloride salt stress treatment. We identified five photosynthesis proteins including Rubisco small subunit, uncharacterized protein LOC100194054, Cyt b6-f, oxygen-evolving enhancer 2, and photosystem I reaction center subunit IV which were significantly up-or down-regulated by salt stress in CW ecotype but not SL population. Gene ontology enrichment analysis showed that photosynthesis was over-represented. The mass spectrometry proteomics data were deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD008482. Taken together, our proteomic study identified differentially accumulated proteins which provide additional evidence of ecophysiological variation in two natural variants of I. cylindrica. ? 2018, College of Life Science. All rights reserved.
URI: https://scholars.lib.ntu.edu.tw/handle/123456789/414125
ISSN: 0372333X
DOI: 10.6165/tai.2018.63.171
Appears in Collections:植物科學研究所

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臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

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