https://scholars.lib.ntu.edu.tw/handle/123456789/414154
標題: | Molecular cloning and photoperiod-regulated expression of gibberellin 20-oxidase from the long-day plant spinach | 作者: | Wu K. Li L. Gage D.A. Zeevaart J.A.D. |
公開日期: | 1996 | 卷: | 110 | 期: | 2 | 起(迄)頁: | 547-554 | 來源出版物: | Plant Physiology | 摘要: | Spinach (Spinacia oleracea L.) is a long-day (LD) rosette plant in which stem growth under LD conditions is mediated by gibberellins (GAs). Major control points in spinach are the later steps of sequential oxidation and elimination of C-20 of C 20 -GAs. Degenerate oligonucleotide primers were used to obtain a polymerase chain reaction product from spinach genomic DNA that has a high homology with GA 20-oxidase cDNAs from Cucurbits maxima L. and Arabidopsis thaliana Heynh. This polymerase chain reaction product was used as a probe to isolate a full-length cDNA clone with an open reading frame encoding a putative 43-kD protein of 374 amino acid residues. When this cDNA clone was expressed in Escherichia coli, the fusion protein catalyzed the biosynthetic sequence GA 53 ¡÷ GA 44 ¡÷ GA 19 ¡÷ GA 20 and GA 19 ¡÷ GA 17 . This establishes that in spinach a single protein catalyzes the oxidation and elimination of C-20. Transfer of spinach plants from short day (SD) to LD conditions caused an increase in the level of all GAs of the early-13-hydroxylation pathway, except GA 53 , with GA 20 , GA 1 , and GA 8 showing the largest increases. Northern blot analysis indicated that the level of GA 20-oxidase mRNA was higher in plants in LD than in SD conditions, with highest level of expression in the shoot tips and elongating stems. This expression pattern of GA 20-oxidase is consistent with the different levels of GA 20 , GA 1 , and GA 8 found in spinach plants grown in SD and LD conditions. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/414154 | ISSN: | 00320889 | DOI: | 10.1104/pp.110.2.547 https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030087737&doi=10.1104%2fpp.110.2.547&partnerID=40&md5=5798d89b77c80f0d3ff0ca61c55729f5 |
SDG/關鍵字: | Cucurbita maxima; Spinacia oleracea |
顯示於: | 植物科學研究所 |
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547.full.pdf | 1.97 MB | Adobe PDF | 檢視/開啟 |
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