https://scholars.lib.ntu.edu.tw/handle/123456789/414156
標題: | Functional analysis of a RPD3 histone deacetylase homologue in Arabidopsis thaliana | 作者: | Wu K. Malik K. Tian L. Brown D. Miki B. |
關鍵字: | Arabidopsis;Gene repression;Histone deacetylase;RPD3 | 公開日期: | 2000 | 卷: | 44 | 期: | 2 | 起(迄)頁: | 167-176 | 來源出版物: | Plant Molecular Biology | 摘要: | Histone acetylation is modulated through the action of histone acetyltransferase and deacetylase, which play key roles in the regulation of eukaryotic gene expression. We have screened the expressed sequence tag database with the yeast histone deacetylase RPD3 sequence and identified two Arabidopsis homologues, AtRPD3A and AtRPD3B. The deduced amino acid sequences of A tRPD3A and AtRPD3B show high overall homology (55% identity) to each other. AtRPD3A encodes a putative protein of 502 amino acids with 49% identity to the yeast RPD3. AtRPD3B encodes a putative protein o f 471 amino acids and shares 55% amino acid identity with the yeast RPD3. Northern analysis indicated that AtRPD3A was highly expressed in the leaves, stems, flowers and young siliques of Arabidopsis plants, whereas the AtRPD3B transcript was not detected in these organs. An AtRPD3A fusion protein repressed transcription when directed to a promoter driving a reporter gene, indicating a role for AtRPD3A protein in gene repression. Arabidopsis plants were transformed with a gene construct comprising a truncated AtRPD3A cDNA in the antisense orientation driven by a strong constitutive promoter, -394tCUP. Antisense expression of AtRPD3A resulted in decreased endogenous AtRPD3A transcript and delayed flowering in transgenic Arabidopsis plants, suggesting that the transition from the vegetative to reproductive phase of development could be affected by histone acetylation. Our study demonstrates the important role of histone deacetylases in plant growth and development. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/414156 | ISSN: | 01674412 | DOI: | 10.1023/A:1006498413543 |
顯示於: | 植物科學研究所 |
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