https://scholars.lib.ntu.edu.tw/handle/123456789/414192
標題: | Genome-wide analysis of MicroRNAs and their target genes related to leaf senescence of rice | 作者: | Xu X. Bai H. Liu C. Chen E. Chen Q. Zhuang J. Shen B. Wu K. |
公開日期: | 2014 | 卷: | 9 | 期: | 12 | 來源出版物: | PLoS ONE | 摘要: | Grain production of rice (Oryza sativa L.) is a top priority in ensuring food security for human beings. One of the approaches to increase yield is to delay leaf senescence and to extend the available time for photosynthesis. MicroRNAs (miRNAs) are key regulators of aging and cellular senescence in eukaryotes. Here, to help understand their biological role in rice leaf senescence, we report identification of miRNAs and their putative target genes by deep sequencing of six small RNA libraries, six RNA-seq libraries and two degradome libraries from the leaves of two super hybrid rice, Nei-2-You 6 (N2Y6, age-resistant rice) and Liang- You-Pei 9 (LYP9, age-sensitive rice). In total 372 known miRNAs, 162 miRNA candidates and 1145 targets were identified. Compared with the expression of miRNAs in the leaves of LYP9, the numbers of miRNAs up-regulated and downregulated in the leaves of N2Y6 were 47 and 30 at early stage of grain-filling, 21 and 17 at the middle stage, and 11 and 37 at the late stage, respectively. Six miRNA families, osa-miR159, osa-miR160 osa-miR164, osa-miR167, osa-miR172 and osa-miR1848, targeting the genes encoding APETALA2 (AP2), zinc finger proteins, salicylic acid-induced protein 19 (SIP19), auxin response factors (ARF) and NAC transcription factors, respectively, were found to be involved in leaf senescence through phytohormone signaling pathways. These results provided valuable information for understanding the miRNA-mediated leaf senescence of rice, and offered an important foundation for rice breeding. ? 2014 Xu et al. Grain production of rice (Oryza sativa L.) is a top priority in ensuring food security for human beings. One of the approaches to increase yield is to delay leaf senescence and to extend the available time for photosynthesis. MicroRNAs (miRNAs) are key regulators of aging and cellular senescence in eukaryotes. Here, to help understand their biological role in rice leaf senescence, we report identification of miRNAs and their putative target genes by deep sequencing of six small RNA libraries, six RNA-seq libraries and two degradome libraries from the leaves of two super hybrid rice, Nei-2-You 6 (N2Y6, age-resistant rice) and Liang- You-Pei 9 (LYP9, age-sensitive rice). In total 372 known miRNAs, 162 miRNA candidates and 1145 targets were identified. Compared with the expression of miRNAs in the leaves of LYP9, the numbers of miRNAs up-regulated and downregulated in the leaves of N2Y6 were 47 and 30 at early stage of grain-filling, 21 and 17 at the middle stage, and 11 and 37 at the late stage, respectively. Six miRNA families, osa-miR159, osa-miR160 osa-miR164, osa-miR167, osa-miR172 and osa-miR1848, targeting the genes encoding APETALA2 (AP2), zinc finger proteins, salicylic acid-induced protein 19 (SIP19), auxin response factors (ARF) and NAC transcription factors, respectively, were found to be involved in leaf senescence through phytohormone signaling pathways. These results provided valuable information for understanding the miRNA-mediated leaf senescence of rice, and offered an important foundation for rice breeding. © 2014 Xu et al. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/414192 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0114313 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84916876947&doi=10.1371%2fjournal.pone.0114313&partnerID=40&md5=fbb734c47f15195403d9c21d332d72ff |
SDG/關鍵字: | auxin response factor; microRNA; microRNA 159; microRNA 160; microRNA 164; microRNA 167; microRNA 172; microRNA 1848; phytohormone; protein APETALA2; protein NAC; salicylic acid induced protein 19; transcription factor; unclassified drug; vegetable protein; zinc finger protein; microRNA; plant RNA; vegetable protein; Article; controlled study; down regulation; gene expression regulation; gene function; gene library; gene targeting; genetic association; genome analysis; grain filling; hybrid; leaf senescence; nonhuman; plant breeding; rice; sequence alignment; sequence analysis; signal transduction; strain difference; upregulation; biosynthesis; genetics; human; metabolism; Oryza; physiology; plant leaf; Eukaryota; Oryza sativa; Osa; Gene Expression Regulation, Plant; Genome-Wide Association Study; Humans; MicroRNAs; Oryza; Plant Leaves; Plant Proteins; RNA, Plant |
顯示於: | 植物科學研究所 |
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journal.pone.0114313 (1).PDF | 2.58 MB | Adobe PDF | 檢視/開啟 |
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