https://scholars.lib.ntu.edu.tw/handle/123456789/414228
標題: | Antibodies against nonstructural protein 1 protect mice from dengue virus-induced mast cell activation | 作者: | Chu, Ya-Ting Wan, Shu-Wen Chang, Yu-Chang CHIEN-KUO LEE BETTY AN-YE WU-HSIEH Anderson, Robert Lin, Yee-Shin |
公開日期: | 27-二月-2017 | 出版社: | NATURE PUBLISHING GROUP | 卷: | 97 | 期: | 5 | 起(迄)頁: | 602 | 來源出版物: | Laboratory investigation; a journal of technical methods and pathology | 摘要: | Dengue virus (DENV) infection causes dengue fever, dengue hemorrhagic fever (DHF), and dengue shock syndrome (DSS). DHF/DSS patients have been reported to have increased levels of urinary histamine, chymase, and tryptase, which are major granule-associated mediators from mast cells. Previous studies also showed that DENV-infected human mast cells induce production of proinflammatory cytokines and chemokines, suggesting a role played by mast cells in vascular perturbation as well as leukocyte recruitment. In this study, we show that DENV but not UV-inactivated DENV enhanced degranulation of mast cells and production of chemokines (MCP-1, RANTES, and IP-10) in a mouse model. We have previously shown that antibodies (Abs) against a modified DENV nonstructural protein 1 (NS1), designated DJ NS1, provide protection in mice against DENV challenge. In the present study, we investigate the effects of DJ NS1 Abs on mast cell-associated activities. We showed that administration of anti-DJ NS1 Abs into mice resulted in a reduction of mast cell degranulation and macrophage infiltration at local skin DENV infection sites. The production of DENV-induced chemokines (MCP-1, RANTES, and IP-10) and the percentages of tryptase-positive activated mast cells were also reduced by treatment with anti-DJ NS1 Abs. These results indicate that Abs against NS1 protein provide multiple therapeutic benefits, some of which involve modulating DENV-induced mast cell activation.Laboratory Investigation advance online publication, 27 February 2017; doi:10.1038/labinvest.2017.10. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019569366&doi=10.1038%2flabinvest.2017.10&partnerID=40&md5=16577ddc678af64ecde61e6595b04024 https://scholars.lib.ntu.edu.tw/handle/123456789/414228 |
ISSN: | 0023-6837 | DOI: | 10.1038/labinvest.2017.10 | SDG/關鍵字: | antibody; chemokine; gamma interferon inducible protein 10; macrophage inflammatory protein 1alpha; macrophage inflammatory protein 1beta; monocyte chemotactic protein 1; nonstructural protein 1; RANTES; tryptase; animal cell; animal experiment; animal model; Article; cell activation; cell infiltration; controlled study; dengue; Dengue virus; enzyme linked immunosorbent assay; immunofluorescence; inoculation; macrophage; mast cell; mast cell degranulation; mouse; nonhuman; priority journal; virus inactivation; virus replication |
顯示於: | 免疫學研究所 |
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