https://scholars.lib.ntu.edu.tw/handle/123456789/414236
標題: | A salivary protein of Aedes aegypti promotes dengue-2 virus replication and transmission | 作者: | Sri-In, Chalida Weng, Shih-Che Chen, Wen-Yu BETTY AN-YE WU-HSIEH Tu, Wu-Chun SHIN-HONG SHIAO |
關鍵字: | Aedes aegypti; Dengue virus; Mosquito salivary proteins; Stat1(−/−) mice; Transmission;Aedes aegypti; Dengue virus; Mosquito salivary proteins; Stat1?/? mice; Transmission | 公開日期: | 八月-2019 | 卷: | 111 | 來源出版物: | Insect biochemistry and molecular biology | 摘要: | Although dengue is the most prevalent arthropod-borne viral disease in humans, no effective medication or vaccine is presently available. Previous studies suggested that mosquito salivary proteins influence infection by the dengue virus (DENV) in the mammalian host. However, the effects of salivary proteins on DENV replication within the Aedes aegypti mosquito remain largely unknown. In this study, we investigated the effect of a specific salivary protein (named AaSG34) on DENV serotype 2 (DENV2) replication and transmission. We showed that transcripts of AaSG34 were upregulated in the salivary glands of Aedes aegypti mosquitoes after a meal of blood infected with DENV2. Transcripts of the dengue viral genome and envelop protein in the salivary glands were significantly diminished after an infectious blood meal when AaSG34 was silenced. The effect of AaSG34 on DENV2 transmission was investigated in Stat1-deficient mice. The intradermal inoculation of infectious mosquito saliva induced hemorrhaging in the Stat1-deficient mice; however, saliva from the AaSG34-silenced mosquitoes did not induce hemorrhaging, suggesting that AaSG34 enhances DENV2 transmission. This is the first report to demonstrate that the protein AaSG34 promotes DENV2 replication in mosquito salivary glands and enhances the transmission of the virus to the mammalian host. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85068213840&doi=10.1016%2fj.ibmb.2019.103181&partnerID=40&md5=4528e132d2e6d26b387d5d1949df7fdb https://scholars.lib.ntu.edu.tw/handle/123456789/414236 |
ISSN: | 09651748 | DOI: | 10.1016/j.ibmb.2019.103181 | SDG/關鍵字: | insect protein; saliva protein; Aedes; animal; C57BL mouse; dengue; Dengue virus; female; growth, development and aging; knockout mouse; mosquito vector; pathology; physiology; RNA interference; virology; virus replication; Aedes; Animals; Dengue; Dengue Virus; Female; Insect Proteins; Mice, Inbred C57BL; Mice, Knockout; Mosquito Vectors; RNA Interference; Salivary Proteins and Peptides; Virus Replication |
顯示於: | 免疫學研究所 |
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