https://scholars.lib.ntu.edu.tw/handle/123456789/414242
標題: | Herpes simplex virus-1 induces expression of a novel MxA isoform that enhances viral replication | 作者: | CHIA-CHI KU Che, Xi-Bing Reichelt, Mike Rajamani, Jaya Schaap-Nutt, Anne Huang, Ke-Jung Sommer, Marvin H Chen, Yi-Shun Chen, Yi-Yuan Arvin, Ann M |
關鍵字: | herpes simplex virus-1 | IFN-α/β | immune evasion | mRNA alternative splicing | 公開日期: | 二月-2011 | 出版社: | WILEY | 卷: | 89 | 期: | 2 | 起(迄)頁: | 173 | 來源出版物: | Immunology and cell biology | 摘要: | MxA is an antiviral protein induced by interferon (IFN)-α/β that is known to inhibit the replication of many RNA viruses. In these experiments, the 76-kDa MxA protein expressed in IFN-α-treated cells was shown to have antiviral activity against herpes simplex virus-1 (HSV-1), a human DNA virus. However, MxA was expressed as a 56-kDa protein in HSV-1-infected cells in the absence of IFN-α. This previously unrecognized MxA isoform was produced from an alternatively spliced MxA transcript that had a deletion of Exons 14-16 and a frame shift altering the C-terminus. The variant MxA (varMxA) isoform was associated with HSV-1 regulatory proteins and virions in nuclear replication compartments. varMxA expression enhanced HSV-1 infection as shown by a reduction in infectious virus titers from cells in which MxA had been inhibited by RNA interference and by an increase in HSV-1 titers when the 56-kDa varMxA was expressed constitutively. Thus, the human MxA gene encodes two MxA isoforms, which are expressed differentially depending on whether the stimulus is IFN-α or HSV-1. These findings show that alternative splicing of cellular mRNA can result in expression of a novel isoform of a host defense gene that supports instead of restricting viral infection. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951947294&doi=10.1038%2ficb.2010.83&partnerID=40&md5=a8a8499e55331bd495e7a0de34c5fc9e https://scholars.lib.ntu.edu.tw/handle/123456789/414242 |
ISSN: | 08189641 | DOI: | 10.1038/icb.2010.83 |
顯示於: | 免疫學研究所 |
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