以SSR分子標幟分析高粱種原之遺傳歧異度
Journal
台灣農學會報
Journal Volume
16卷
Journal Issue
1
Pages
85-110
Date Issued
2015
Author(s)
Abstract
Sorghum [Sorghum bicolor (L.) Moench, 2n = 2x = 20] is the fifth most important cereal crop in the world. Because of its tolerance to long-term drought and barren soil among the cereal crops of Poaceae, sorghum is suitable for cultivation by low input, resulting in being as a famine crop. In addition, sorghum has multipurpose-utilization such as forage, broom, brewing and bio-ethanol production. To assess the genetic diversity of sorghum germplasm is essential for breeding and genetic researches, 53 markers of simple sequence repeats, SSRs, were employed to genotype 93 sorghum germplasm which were obtained from National Plant Genetic Resources Center and collected from fields in Taiwan. The average number of allele detected by each marker was 14.4, and the polymorphic information content (PIC) ranged from 0.206 to 0.922 with a mean of 0.709, indicating high discriminating ability of SSRs. The 93 germplasm could be divided into eight subpopulations and three major clusters with several minor groups revealed by STRUCTRE and cladogram of Unweighted Pair Group Method with Arithmetic Mean (UPGMA) based on Nei's genetic distances. Both STRUCTURE and UPGMA revealed congruent clusters, for which S. propinquum and S. halepense , Texas accessions and accessions prefixed V, part of introduced germplasm, Taiwanese accessions, and breeding lines of Hengchun Branch, Livestock Research Institute were grouped in either the same subpopulation or minor clusters, respectively. This study paves a way for researches of sorghum genetics and breeding in Taiwan.
Subjects
Genetic diversity
Molecular marker
Population Structure
Simple sequence repeat (SSR)
Sorghum
Type
journal article