Carbon monoxide regulates the expression of the wound-inducible gene ipomoelin through antioxidation and MAPK phosphorylation in sweet potato
Journal
Journal of Experimental Botany
Journal Volume
65
Journal Issue
18
Pages
5279
Date Issued
2014-10-01
Author(s)
Lin, Jeng Shane
Lin, Hsin Hung
Li, Yu Chi
King, Yu Chi
Sung, Ruei Jin
Kuo, Yun Wei
Lin, Chih Ching
Shen, Yu Hsing
Abstract
© The Author 2014. Carbon monoxide (CO), one of the haem oxygenase (HO) products, plays important roles in plant development and stress adaptation. However, the function of CO involved in wounding responses is seldom studied. A wound-inducible gene, ipomoelin (IPO), of sweet potato (Ipomoea batatas cv. Tainung 57) was used as a target to study the regulation of CO in wounding responses. After wounding for 1 h, the endogenous CO content and IbHO expression level were significantly reduced in leaves. IPO expression upon wounding was prohibited by the HO activator hemin, whereas the HO inhibitor zinc protoporphyrin IX elevated IPO expression. The IPO expression induced by wounding, H2O2, or methyl jasmonate was inhibited by CO. CO also affected the activities of ascorbate peroxidase, catalase, and peroxidase, and largely decreased H2O2 content in leaves. CO inhibited the extracellular signal-regulated kinase (ERK) phosphorylation induced by wounding. IbMAPK, the ERK of sweet potato, was identified by immunoblotting, and the interaction with its upstream activator, IbMEK1, was further confirmed by bimolecular fluorescence complementation and co-immunoprecipitation. Conclusively, wounding in leaves repressed IbHO expression and CO production, induced H2O2 generation and ERK phosphorylation, and then stimulated IPO expression.
Subjects
Carbon monoxide | ERK phosphorylation | H<inf>2</inf>O<inf>2</inf> | Ipomoelin | Sweet potato | Wounding
SDGs
Publisher
OXFORD UNIV PRESS
Type
journal article
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