https://scholars.lib.ntu.edu.tw/handle/123456789/414341
標題: | UBC18 mediates ERF1 degradation under light–dark cycles | 作者: | Cheng, Mei Chun Kuo, Wen Chieh Wang, Yi Ming Chen, Hsing Yu TSAN-PIAO LIN |
關鍵字: | abiotic stress | degradation | E2 | Ethylene Response Factor 1 (ERF1) | proline | UBIQUITIN-CONJUGATING ENZYME 18 (UBC18) | ubiquitination | 公開日期: | 1-二月-2017 | 出版社: | WILEY-BLACKWELL | 卷: | 213 | 期: | 3 | 起(迄)頁: | 1156 | 來源出版物: | New Phytologist | 摘要: | © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust Ethylene Response Factor 1 (ERF1) plays a crucial role in biotic and abiotic stress responses. Previous studies have shown that ERF1 regulates stress-responsive gene expression by binding to different cis-acting elements in response to various stress signals. ERF1 was also reported to be unstable in the dark, and it regulates hypocotyl elongation. Here, we elucidated the mechanism underlying degradation of ERF1. Yeast two-hybrid screening showed that UBIQUITIN-CONJUGATING ENZYME 18 (UBC18) interacted with ERF1. The interaction between ERF1 and UBC18 was verified using pull-down assays and coimmunoprecipitation analyses. We then compared the ERF1 protein abundance in the UBC18 mutant and overexpression plants. Based on the results of protein degradation and in vivo ubiquitination assays, we proposed that UBC18 mediates ERF1 ubiquitination and degradation. ERF1 was more stable in UBC18 mutants and less stable in UBC18 overexpression lines compared with that in wild-type plants. ERF1 was degraded by the 26S proteasome system via regulation of UBC18 and promotes dark-repression of downstream genes and proline accumulation. UBC18 negatively regulated drought and salt stress responses by altering the abundance of ERF1 and the expression of genes downstream of ERF1. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/414341 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84998910068&doi=10.1111%2fnph.14272&partnerID=40&md5=174103e746d994fa1a856aaa4456e583 |
ISSN: | 0028646X | DOI: | https://api.elsevier.com/content/abstract/scopus_id/84998910068 10.1111/nph.14272 |
顯示於: | 植物科學研究所 |
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