https://scholars.lib.ntu.edu.tw/handle/123456789/414345
標題: | The heat stress factor HSFA6b connects ABA signaling and ABA-mediated heat responses | 作者: | Huang, Ya Chen Niu, Chung Yen Yang, Chen Ru TSUNG-LUO JINN |
公開日期: | 1-十月-2016 | 出版社: | AMER SOC PLANT BIOLOGISTS | 卷: | 172 | 期: | 2 | 起(迄)頁: | 1182 | 來源出版物: | Plant Physiology | 摘要: | © 2016 American Society of Plant Biologists. All rights reserved. Heat stress response (HSR) is a conserved mechanism developed to increase the expression of heat shock proteins (HSPs) via a heat shock factor (HSF)-dependent mechanism. Signaling by the stress phytohormone abscisic acid (ABA) is involved in acquired thermotolerance as well. Analysis of Arabidopsis (Arabidopsis thaliana) microarray databases revealed that the expression of HSFA6b, a class A HSF, extensively increased with salinity, osmotic, and cold stresses, but not heat. Here, we show that HSFA6b plays a pivotal role in the response to ABA and in thermotolerance. Salt-inducible HSFA6b expression was down-regulated in ABA-insensitive and -deficient mutants; however, exogenous ABA application restored expression in ABAdeficient, but not -insensitive plants. Thus, ABA signaling is required for proper HSFA6b expression. A transcriptional activation assay of protoplasts revealed that ABA treatment and coexpression of an ABA signaling master effector, ABA-RESPONSIVE ELEMENT-BINDING PROTEIN1, could activate the HSFA6b promoter. In addition, HSFA6b directly bound to the promoter of DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2A and enhanced its expression. Analysis of ABA responses in seed germination, cotyledon greening, and root growth as well as salt and drought tolerance in HSFA6b-null, overexpression, and dominant negative mutants revealed that HSFA6b is a positive regulator participating in ABA-mediated salt and drought resistance. Thermoprotection tests showed that HSFA6b was required for thermotolerance acquisition. Our study reveals a network in which HSFA6b operates as a downstream regulator of the ABA-mediated stress response and is required for heat stress resistance. This new ABA-signaling pathway is integrated into the complex HSR network in planta. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/414345 | ISSN: | 00320889 | DOI: | https://api.elsevier.com/content/abstract/scopus_id/84989306794 10.1104/pp.16.00860 |
顯示於: | 植物科學研究所 |
檔案 | 描述 | 大小 | 格式 | |
---|---|---|---|---|
1182.full.pdf | 2.69 MB | Adobe PDF | 檢視/開啟 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。