|Title:||Heat shock-induced biphasic Ca2+ signature and OsCaM1-1 nuclear localization mediate downstream signalling in acquisition of thermotolerance in rice (Oryza sativa L.)||Authors:||Wu, Hui Chen
Luo, Dan Li
|Keywords:||Apoplastic Ca 2+ | Ca signalling 2+ | Calmodulin | Heat shock protein | Heat shock signalling||Issue Date:||1-Sep-2012||Publisher:||WILEY||Journal Volume:||35||Journal Issue:||9||Start page/Pages:||1543||Source:||Plant, Cell and Environment||Abstract:||
We investigated heat shock (HS)-triggered Ca2+ signalling transduced by a Ca2+ sensor, calmodulin (CaM), linked to early transcriptome changes of HS-responsive genes in rice. We observed a biphasic [Ca2+]cyt signature in root cells that was distinct from that in epicotyl and leaf cells, which showed a monophasic response after HS. Treatment with Ca2+ and A23187 generated an intense and sustained increase in [Ca2+]cyt in response to HS. Conversely, treatment with Ca2+ chelator, L-type Ca2+ channel blocker and CaM antagonist, but not intracellular Ca2+ release inhibitor, strongly inhibited the increased [Ca2+]cyt. HS combined with Ca2+ and A23187 accelerated the expression of OsCaM1-1 and sHSPC/N genes, which suggests that the HS-induced apoplastic Ca2+ influx is responsible for the [Ca2+]cyt response and downstream HS signalling. In addition, the biphasic response of OsCaM1-1 in the nucleus followed the Ca2+ signature, which may provide the information necessary to direct HS-related gene expression. Overexpression of OsCaM1-1 induced the expression of Ca2+/HS-related AtCBK3, AtPP7, AtHSF and AtHSP at a non-inducing temperature and enhanced intrinsic thermotolerance in transgenic Arabidopsis. Therefore, HS-triggered rapid increases in [Ca2+]cyt, together with OsCaM1-1 expression and its nuclear localization, are important in mediating downstream HS-related gene expression for the acquisition of thermotolerance in rice. © 2012 Blackwell Publishing Ltd.
|Appears in Collections:||植物科學研究所|
|j.1365-3040.2012.02508.x.pdf||2.38 MB||Adobe PDF||View/Open|
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