DC 欄位 | 值 | 語言 |
dc.contributor.author | Wu, Hui Chen | en_US |
dc.contributor.author | Luo, Dan Li | en_US |
dc.contributor.author | Vignols, Florence | en_US |
dc.contributor.author | TSUNG-LUO JINN | en_US |
dc.creator | TSUNG-LUO JINN;Vignols, Florence;Luo, Dan Li;Wu, Hui Chen | - |
dc.date.accessioned | 2019-07-22T02:59:10Z | - |
dc.date.available | 2019-07-22T02:59:10Z | - |
dc.date.issued | 2012-09-01 | - |
dc.identifier.issn | 01407791 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/414352 | - |
dc.description.abstract | We investigated heat shock (HS)-triggered Ca2+ signalling transduced by a Ca2+ sensor, calmodulin (CaM), linked to early transcriptome changes of HS-responsive genes in rice. We observed a biphasic [Ca2+]cyt signature in root cells that was distinct from that in epicotyl and leaf cells, which showed a monophasic response after HS. Treatment with Ca2+ and A23187 generated an intense and sustained increase in [Ca2+]cyt in response to HS. Conversely, treatment with Ca2+ chelator, L-type Ca2+ channel blocker and CaM antagonist, but not intracellular Ca2+ release inhibitor, strongly inhibited the increased [Ca2+]cyt. HS combined with Ca2+ and A23187 accelerated the expression of OsCaM1-1 and sHSPC/N genes, which suggests that the HS-induced apoplastic Ca2+ influx is responsible for the [Ca2+]cyt response and downstream HS signalling. In addition, the biphasic response of OsCaM1-1 in the nucleus followed the Ca2+ signature, which may provide the information necessary to direct HS-related gene expression. Overexpression of OsCaM1-1 induced the expression of Ca2+/HS-related AtCBK3, AtPP7, AtHSF and AtHSP at a non-inducing temperature and enhanced intrinsic thermotolerance in transgenic Arabidopsis. Therefore, HS-triggered rapid increases in [Ca2+]cyt, together with OsCaM1-1 expression and its nuclear localization, are important in mediating downstream HS-related gene expression for the acquisition of thermotolerance in rice. © 2012 Blackwell Publishing Ltd. | en_US |
dc.language.iso | en | en_US |
dc.publisher | WILEY | en_US |
dc.relation.ispartof | Plant, Cell and Environment | en_US |
dc.subject | Apoplastic Ca 2+ | Ca signalling 2+ | Calmodulin | Heat shock protein | Heat shock signalling | en_US |
dc.title | Heat shock-induced biphasic Ca2+ signature and OsCaM1-1 nuclear localization mediate downstream signalling in acquisition of thermotolerance in rice (Oryza sativa L.) | en_US |
dc.type | journal article | en |
dc.identifier.doi | https://api.elsevier.com/content/abstract/scopus_id/84864835501 | - |
dc.identifier.doi | 10.1111/j.1365-3040.2012.02508.x | - |
dc.identifier.pmid | 22428987 | - |
dc.identifier.scopus | 2-s2.0-84864835501 | - |
dc.identifier.isi | WOS:000307386800002 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/84864835501 | - |
dc.relation.pages | 1543 | en_US |
dc.relation.journalvolume | 35 | en_US |
dc.relation.journalissue | 9 | en_US |
dc.relation.pageend | 1557 | en_US |
item.openairetype | journal article | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | with fulltext | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
crisitem.author.dept | Plant Biology | - |
crisitem.author.dept | Life Science | - |
crisitem.author.dept | Ph. D. Program In Plant Biotechnology Development Of Industry-academia Cooperation | - |
crisitem.author.orcid | 0000-0002-5185-7691 | - |
crisitem.author.parentorg | College of Life Science | - |
crisitem.author.parentorg | College of Life Science | - |
crisitem.author.parentorg | College of Life Science | - |
顯示於: | 植物科學研究所
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