https://scholars.lib.ntu.edu.tw/handle/123456789/414761
標題: | Role of Penaeus monodon Kruppel-like factor (PmKLF) in infection by white spot syndrome virus | 作者: | L. K. Chang P. H. Huang W. T. Shen S. H. Yang W. J. Liu C. F. Lo |
關鍵字: | Kruppel-like factor; Penaeus monodon; White spot syndrome virus | 公開日期: | 2012 | 卷: | 36 | 期: | 1 | 起(迄)頁: | 121-129 | 來源出版物: | Developmental and Comparative Immunology | 摘要: | Sp1-like proteins and Kruppel-like factors (KLFs) are highly related zinc-finger proteins that have crucial roles in transcription. One expressed sequence tag (EST, HPA-N-S01-EST0038) from shrimps is homologous to Sp1. This study reports the cloning and characteristics of a KLF from shrimp, Penaeus monodon (PmKLF). The full-length PmKLF cDNA is 1702. bp, encoding a polypeptide of 360 amino acids. Sequence analysis revealed that the sequence of PmKLF is similar to that of KLF11 in humans, mice and zebrafish. RT-PCR analysis indicated that PmKLF mRNA is expressed in all examined tissues. Additionally, immunofluorescence analysis revealed that GFP-KLF fusion protein is located in the nucleus as dots in an insect cell line, Sf9. Localization of PmKLF in the nucleus is also observed in the hemolymph from white spot syndrome virus (WSSV)-infected and WSSV-uninfected Litopenaeus vannamei. Knockdown of the expression of PmKLF transcript in WSSV-infected shrimp resulted in delayed cumulative mortalities, suggesting that PmKLF is important to WSSV infection. Moreover, inhibition of PmKLF expression reduced the copy number of WSSV and ie1 expression, revealing that PmKLF affects WSSV infection via interfering with ie1 expression. ? 2011 Elsevier Ltd. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/414761 | ISSN: | 0145 305X | DOI: | 10.1016/j.dci.2011.06.008 | SDG/關鍵字: | complementary DNA; kruppel like factor; kruppel like factor 11; messenger RNA; RNA 16S; transcription factor Sp1; unclassified drug; amino acid sequence; animal tissue; article; cell nucleus; cellular distribution; controlled study; gene; gene expression; gene silencing; hemolymph; nonhuman; nucleotide sequence; PmKLF gene; priority journal; protein expression; protein function; protein localization; reverse transcription polymerase chain reaction; sequence analysis; sequence homology; shrimp; White spot syndrome virus; Animals; Base Sequence; Cell Cycle Proteins; Cell Line; Cloning, Molecular; DNA Virus Infections; Gene Expression Regulation, Viral; Gene Knockdown Techniques; Hemolymph; Humans; Insects; Kruppel-Like Transcription Factors; Mice; Molecular Sequence Data; Penaeidae; Repressor Proteins; Sp1 Transcription Factor; Viral Proteins; White spot syndrome virus 1; Danio rerio; Decapoda (Crustacea); Hexapoda; Litopenaeus vannamei; Mus; Penaeus monodon; Shrimp white spot syndrome virus |
顯示於: | 生化科技學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。