Generation of FHL2 homozygous knockout lines from human embryonic stem cells by CRISPR/Cas9 mediated ablation
Journal
Stem Cell Research
Journal Volume
27
Pages
21 24
Date Issued
2018
Author(s)
Abstract
Cardiovascular disease is the leading cause of morbidity and mortality in the world. Mutations in the FHL2 (Four and a half LIM domains protein 2) gene are associated with cardiomyopathy in patients. Here, we generated two homozygous knockout lines using CRISPR/Cas9-mediated ablation in a human embryonic stem cell (hESC) WA09 line. These knockout lines exhibit a normal karyotype without expressing FHL2 protein, while maintaining pluripotency and differentiation properties. These isogenic mutation lines will be provided as a disease model for cardiomyopathy studies and drug screening.
SDGs
Type
journal article