https://scholars.lib.ntu.edu.tw/handle/123456789/416267
Title: | SUMOylation attenuates c-Maf-dependent IL-4 expression | Authors: | Lin, Bo-Shiou Tsai, Pei-Yun Hsieh, Wan-Yun Tsao, Hsiao-Wei Liu, Meng-Wei Grenningloh, Roland LI-FANG WANG Ho, I-Cheng SHI-CHUEN MIAW |
Keywords: | c-Maf; Il4 Gene regulation; SUMOylation | Issue Date: | Apr-2010 | Publisher: | WILEY | Journal Volume: | 40 | Journal Issue: | 4 | Start page/Pages: | 1174 | Source: | European journal of immunology | Abstract: | The function of transcription factors can be critically regulated by SUMOylation. c-Maf, the cellular counterpart of v-maf oncogene, is a potent transactivator of the IL-4 gene in Th2 cells. We found in a yeast two-hybrid screen that c-Maf can interact with Ubc9 and PIAS1, two key enzymes of the SUMOylation pathway. In this study, we report that c-Maf co-localized with these two SUMO (small ubiquitin-like modifier) ligases in the nucleus and that c-Maf can be SUMOylated in vitro and also in primary Th2 cells. We also demonstrated that lysine-33 is the dominant, if not the only, SUMO acceptor site of c-Maf. SUMOylation of c-Maf attenuated its transcriptional activity. Reciprocally, a SUMOylation resistant c-Maf was more potent than WT-c-Maf in driving IL-4 production in c-Maf-deficient Th2 cells. Furthermore, we showed that ablation of the SUMO site did not alter the subcellular localization or the stability of c-Maf protein but instead enhanced its recruitment to the Il4-promoter. We conclude that SUMOylation at lysine-33 is a functionally critical post-translational modification event of c-Maf in Th cells. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-77951068773&doi=10.1002%2feji.200939788&partnerID=40&md5=8b8de18bbc4045a511cde56564e3f141 https://scholars.lib.ntu.edu.tw/handle/123456789/416267 |
ISSN: | 0014-2980 | DOI: | 10.1002/eji.200939788 |
Appears in Collections: | 免疫學研究所 |
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