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  1. NTU Scholars
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Please use this identifier to cite or link to this item: https://scholars.lib.ntu.edu.tw/handle/123456789/416270
Title: 14-3-3θ is a binding partner of rat Eag1 potassium channels
Authors: Hsu, Po-Hao
SHI-CHUEN MIAW 
Chuang, Chau-Ching
Chang, Pei-Yu
Fu, Ssu-Ju
Jow, Guey-Mei
Chiu, Mei-Miao
Jeng, Chung-Jiuan
Issue Date: 2012
Publisher: PUBLIC LIBRARY SCIENCE
Journal Volume: 7
Journal Issue: 7
Source: PloS one
Abstract: 
The ether-à-go-go (Eag) potassium (K(+)) channel belongs to the superfamily of voltage-gated K(+) channel. In mammals, the expression of Eag channels is neuron-specific but their neurophysiological role remains obscure. We have applied the yeast two-hybrid screening system to identify rat Eag1 (rEag1)-interacting proteins from a rat brain cDNA library. One of the clones we identified was 14-3-3θ, which belongs to a family of small acidic protein abundantly expressed in the brain. Data from in vitro yeast two-hybrid and GST pull-down assays suggested that the direct association with 14-3-3θ was mediated by both the N- and the C-termini of rEag1. Co-precipitation of the two proteins was confirmed in both heterologous HEK293T cells and native hippocampal neurons. Electrophysiological studies showed that over-expression of 14-3-3θ led to a sizable suppression of rEag1 K(+) currents with no apparent alteration of the steady-state voltage dependence and gating kinetics. Furthermore, co-expression with 14-3-3θ failed to affect the total protein level, membrane trafficking, and single channel conductance of rEag1, implying that 14-3-3θ binding may render a fraction of the channel locked in a non-conducting state. Together these data suggest that 14-3-3θ is a binding partner of rEag1 and may modulate the functional expression of the K(+) channel in neurons.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864098814&doi=10.1371%2fjournal.pone.0041203&partnerID=40&md5=be82d3ab73081591794e4312397d4957
https://scholars.lib.ntu.edu.tw/handle/123456789/416270
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0041203
Appears in Collections:免疫學研究所

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臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

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