Repository logo
  • English
  • 中文
Log In
Have you forgotten your password?
  1. Home
  2. College of Medicine / 醫學院
  3. Microbiology / 微生物學科所
  4. Activation of mitogen-activated protein kinases is essential for hydrogen peroxide -induced apoptosis in retinal pigment epithelial cells
 
  • Details

Activation of mitogen-activated protein kinases is essential for hydrogen peroxide -induced apoptosis in retinal pigment epithelial cells

Journal
Apoptosis
Journal Volume
11
Journal Issue
11
Pages
1899-1908
Date Issued
2006
Author(s)
Ho T.-C.
Yang Y.-C.
Cheng H.-C.
Wu A.-C.
SHOW-LI CHEN  
Chen H.-K.
Tsao Y.-P.
DOI
10.1007/s10495-006-9403-6
URI
https://www.scopus.com/inward/record.uri?eid=2-s2.0-33750622313&doi=10.1007%2fs10495-006-9403-6&partnerID=40&md5=39e4195f75fd4c36d108f95cbc155633
https://scholars.lib.ntu.edu.tw/handle/123456789/416953
Abstract
Retinal pigment epithelial (RPE) cells are constantly exposed to oxidative injury while clearing byproducts of photoreceptor turnover, a circumstance thought to be responsible for degenerative retinal diseases. The mechanisms of hydrogen peroxide (H2O2)-induced apoptosis in RPE cells are not fully understood. We studied signal transduction mechanisms of H 2O2-induced apoptosis in the human RPE cell line ARPE-19. Activation of two stress kinases (JNK and p38) occurs during H2O 2 stimulation, and H2O2-mediated cell death was significantly reduced by their specific inhibition. Exposure to a lethal dose of H2O2 elicited Bax translocation to the mitochondria and release of apoptosis-inducing factor (AIF) from the mitochondria, both of which were abolished by either JNK- or p38-specific inhibitors. Both H 2O2-induced cell death and JNK/p38 phosphorylation were partially inhibited by C. difficile toxin B, inhibitor of Rho, Rac, and cdc42. Use of pull-down assays revealed that the small GTPase activated by H 2O2 is Rac1. This study is the first to demonstrate that H2O2 induces a Rac1/JNK1/p38 signaling cascade, and that JNK and p38 activation is important for H2O2-induced apoptosis as well as AIF/Bax translocation of RPE cells. ? 2006 Springer Science + Business Media, LLC.
Subjects
AIF; Hydrogen peroxide; JNK; p38; Rac1; RPE
SDGs

[SDGs]SDG3

Other Subjects
apoptosis inducing factor; hydrogen peroxide; mitogen activated protein kinase p38; mitogen activated protein kinase p38 inhibitor; protein Bax; Rac1 protein; stress activated protein kinase; stress activated protein kinase inhibitor; toxin; apoptosis; article; cell death; controlled study; cytokine release; enzyme activation; enzyme phosphorylation; exposure; human; human cell; mitochondrion; pigment cell; pigment epithelium; priority journal; signal transduction; Active Transport, Cell Nucleus; Anthracenes; Apoptosis; Apoptosis Regulatory Proteins; Bacterial Proteins; Bacterial Toxins; Cell Line, Tumor; Cell Nucleus; Enzyme Inhibitors; Humans; Hydrogen Peroxide; Imidazoles; MAP Kinase Signaling System; Mitogen-Activated Protein Kinase 8; p38 Mitogen-Activated Protein Kinases; Pigment Epithelium of Eye; Protein Transport; Pyridines; rac1 GTP-Binding Protein; Clostridium difficile
Type
journal article

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Open policy finder網站查詢,以確認出版單位之版權政策。
    Please use Open policy finder to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science