Human papillomavirus 11 E5A delays the growth restriction induced by temperature shift in temperature-sensitive simian virus 40 T antigen-immortalized keratinocytes
Journal
Biochemical and Biophysical Research Communications
Journal Volume
217
Journal Issue
3
Pages
712-720
Date Issued
1995
Author(s)
Abstract
The transfection with human papillomavirus type 11 E5a oncoprotein can cause longer and more active proliferation in the human keratinocytes previously transfected stably with temperature sensitive SV40 T antigen at 39 degrees C. Also, after the E5a transfection in parental temperature sensitive SV40 T antigen gene transfected cells, we observe a delay in the accumulation of p21 gene at 39 degrees C. Moreover, T antigen degradation did not occur at 39 degrees C in the E5a transfected cells as it did in the parental cells. We draw from these observations that E5a may transiently stabilize temperature sensitive T antigen and/or repress p21 synthesis.
Type
journal article