Construction of site-specific mutants of E5A protein of HPV-11 using the polymerase chain reaction and a single mutant primer
Journal
Journal of Virological Methods
Journal Volume
42
Journal Issue
1
Pages
89-94
Date Issued
1993
Author(s)
Abstract
The method of Perrin and Gilliland (1990) was modified to create site-specific mutants. The polymerase chain reaction and a single mutant primer are needed to carry out site-specific mutagenesis. Using this method, removal of the excess primers and nucleotides from the initial amplification is not necessary. This method provides a simpler way to generate site-specific mutants.
Type
journal article