https://scholars.lib.ntu.edu.tw/handle/123456789/417106
標題: | Epstein-Barr virus nuclear antigen 2 retards cell growth, induces p21(WAF1) expression, and modulates p53 activity post-translationally | 作者: | Lin C.-S. Kuo H.-H. Chen J.-Y. Yang C.-S. WON-BO WANG |
關鍵字: | Epstein-Barr virus (EBV); Growth retardation; Nuclear antigen 2 (EBNA2); P21(WAF1); P53 | 公開日期: | 2000 | 卷: | 303 | 期: | 1 | 起(迄)頁: | 7-23 | 來源出版物: | Journal of Molecular Biology | 摘要: | The Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2) has been shown to be required for promotion of cell-cycle progression in EBV-immortalized B-lymphocytes. However, other studies have indicated that EBNA2 alone, in the absence of other EBV genes, may retard cell growth. To resolve this discrepancy, we investigated the effect of EBNA2 on the growth of various cells, including EBV target nasopharyngeal carcinoma cells, NPC-TW01 and NPC-TW04. We found that EBNA2 could retard cell growth, in stable Vero, HEp-2, and U2OS cell clones expressing EBNA2, and in Vero, 293, NPC-TW01, and NPC-TW04 cells transiently transfected with EBNA2. While investigating the mechanism underlying the growth-retarding function of EBNA2, we found that EBNA2 induced p21(WAF1) expression in these cells. This induction of p21(WAF1) expression was mediated through p53. EBNA2 was found to stimulate p53 to bind to the p53-response element within the p21(WAF1) promoter, possibly by promoting p53 phosphorylation. This enhancement of p53 sequence-specific DNA-binding activity may be the mechanism through which EBNA2 activates the expression of p53-regulated genes, including p21(WAF1) and mdm-2. Together, these studies reveal a possible intrinsic function of EBNA2 in cell-growth regulation and elucidate a novel mechanism by which EBNA2 modulates transcription. (C) 2000 Academic Press. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034644726&doi=10.1006%2fjmbi.2000.4121&partnerID=40&md5=42d77aff3a4dbdcea1cc0de9e16bc6b2 https://scholars.lib.ntu.edu.tw/handle/123456789/417106 |
ISSN: | 0022-2836 | DOI: | 10.1006/jmbi.2000.4121 | SDG/關鍵字: | cell nucleus antigen; protein p21; protein p53; virus antigen; animal cell; article; cancer cell culture; cell growth; Epstein Barr virus; gene expression; growth inhibition; growth regulation; human; human cell; nonhuman; priority journal; protein DNA binding; protein phosphorylation; transcription regulation; Animalia; DNA viruses; Human herpesvirus 4 |
顯示於: | 微生物學科所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。