https://scholars.lib.ntu.edu.tw/handle/123456789/417176
標題: | Serum-induced iron-acquisition systems and TonB contribute to virulence in Klebsiella pneumoniae causing primary pyogenic liver abscess | 作者: | Hsieh P.-F. Lin T.-L. Lee, Cha-Ze Tsai S.-F. JIN-TOWN WANG |
公開日期: | 2008 | 卷: | 197 | 期: | 12 | 起(迄)頁: | 1717-1727 | 來源出版物: | Journal of Infectious Diseases | 摘要: | Background. Klebsiella pneumoniae has become the predominant pathogen causing primary pyogenic liver abscess (PLA). Methods. K. pneumoniae was stimulated by human serum, and gene expression was analyzed by microarray. Results. Three putative iron acquisition systems, Yersinia high-pathogenicity island (HPI), iucABCDiutA, and iroA(iroNDCB), that increased in expression and predominated in PLA-associated K. pneumoniae strains were identified. By use of siderophore uptake assays, these 3 systems were confirmed to be siderophore-dependent iron acquisition systems. Only the irp2-iuc-iroA triple mutant showed decreased virulence in mice. Full-genome analysis of K. pneumoniae strain NTUH-K2044 identified 10 putative iron uptake systems. Seven of these 10 systems were TonB dependent, including Yersinia HPI, iucABCDiutA, and iroA. A tonB deletion mutant was demonstrated to have profound attenuation of virulence. Immunization with the tonB mutant resulted in seroconversion of extracellular polysaccharide antibodies and protective efficacy against subsequent exposure to the parental strain. Conclusions. Iron uptake systems were the genes in K. pneumoniae that were highly up-regulated in response to sera. Although there are multiple iron transporter systems in NTUH-K2044, a mutation in all 3 loci (irp2, iuc, and iroA) is necessary to decrease virulence. The tonB mutant is a potential vaccine candidate because it can induce a significant protective immune response against challenge with a wild-type strain. ? 2008 by the Infectious Diseases Society of America. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-46349110896&doi=10.1086%2f588383&partnerID=40&md5=7b739da00b048f0a8318cd28cbf83606 https://scholars.lib.ntu.edu.tw/handle/123456789/417176 |
ISSN: | 0022-1899 | DOI: | 10.1086/588383 | SDG/關鍵字: | bacterial RNA; iron; siderophore; animal experiment; animal model; article; bacterial gene; bacterial virulence; bacterium isolate; controlled study; DNA microarray; enzyme linked immunosorbent assay; female; gene locus; genotype; iroa gene; iron metabolism; iron transport; irp2 gene; iuc gene; Klebsiella pneumoniae; mouse; nonhuman; nucleotide sequence; priority journal; pyogenic liver abscess; serum; Animals; Bacterial Proteins; Biological Transport, Active; Carrier Proteins; Female; Gene Deletion; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Humans; Iron; Klebsiella Infections; Klebsiella pneumoniae; Liver Abscess, Pyogenic; Membrane Proteins; Mice; Mice, Inbred BALB C; Multigene Family; Mutation; Serum; Transcription, Genetic; Virulence |
顯示於: | 微生物學科所 |
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