https://scholars.lib.ntu.edu.tw/handle/123456789/417205
標題: | CagA tyrosine phosphorylation in gastric epithelial cells caused by Helicobacter pylori in patients with gastric adenocarcinoma | 作者: | YO-PING LAI JYH-CHIN YANG Lin T.-Z. Wang, Jin-Town Lin J.-T. |
公開日期: | 2003 | 卷: | 8 | 期: | 3 | 起(迄)頁: | 235-243 | 來源出版物: | Helicobacter | 摘要: | Background. Tyrosine phosphorylation of Helicobacter pylori cytotoxin-associated protein of in gastric epithelial cells is reported. The goals of this study are first to examine the occurrence of CagA tyrosine phosphorylation in H. pylori strains isolated from patients with gastric adenocarcinoma and gastritis, and second to clarify the relationship between the diversity of tyrosine phosphorylation motifs and the presence of CagA tyrosine phosphorylation. Methods. Fifty-eight clinical isolates of H. pylori from patients with gastric adenocarcinoma (29 cases) and gastritis (29 cases) were studied for CagA tyrosine phosphorylation by Western blotting. Sequence diversity of tyrosine phosphorylation motifs was analysed among positive- or negative-CagA tyrosine phosphorylation isolates. Results. Positive CagA tyrosine phosphorylation was found in 93.1% (27 of 29) of strains from gastric adenocarcinoma patients and 51.7% (15 of 29) of strains from gastritis patients (p < 0.001). Intact motifs were found in H. pylori isolates with CagA tyrosine phosphorylation. Of the 16 negative CagA tyrosine phosphorylation isolates, intact tyrosine phosphorylation motifs were found in 15 isolates. Conclusions. CagA tyrosine phosphorylation, which is significantly greater in strains from gastric adenocarcinoma patients, may play a role in gastric carcinogenesis, and could be a better marker of more virulent strains than the cag pathogenicity island in Asia, where the cag pathogenicity island is present in nearly all H. pylori strains. Sequence diversity of tyrosine phosphorylation motifs on CagA was not related to the presence of tyrosine phosphorylation. The absence of tyrosine phosphorylation motif might result in negative tyrosine phosphorylation phenotypes, but such motifs are not the sole factors associated with CagA tyrosine phosphorylation. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037844366&doi=10.1046%2fj.1523-5378.2003.00148.x&partnerID=40&md5=b1b19012d166a425f4c2f9d0c7124139 https://scholars.lib.ntu.edu.tw/handle/123456789/417205 |
ISSN: | 1083-4389 | DOI: | 10.1046/j.1523-5378.2003.00148.x | SDG/關鍵字: | CagA protein; tyrosine; article; bacterial gene; bacterial strain; bacterium isolate; controlled study; disease association; disease marker; gastritis; gene sequence; genetic variability; Helicobacter pylori; human; human cell; nonhuman; pathogenicity; priority journal; protein motif; protein phosphorylation; stomach adenocarcinoma; stomach carcinogenesis; stomach epithelium; Western blotting; Adenocarcinoma; Amino Acid Sequence; Antigens, Bacterial; Bacterial Proteins; Cells, Cultured; DNA, Bacterial; Epithelial Cells; Helicobacter Infections; Helicobacter pylori; Humans; Molecular Sequence Data; Phosphorylation; Stomach; Stomach Neoplasms; Tyrosine; Variation (Genetics); Bacteria (microorganisms); Helicobacter; Helicobacter pylori |
顯示於: | 微生物學科所 |
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