https://scholars.lib.ntu.edu.tw/handle/123456789/417249
Title: | Direct DNA Amplification and Restriction Pattern Analysis of Helicobacter pylori in Patients with Duodenal Ulcer and Their Families | Authors: | JIN-TOWN WANG Wang T.-H. JIN-CHUAN SHEU Lin J.-T. MING-SHIANG WU |
Issue Date: | 1993 | Journal Volume: | 168 | Journal Issue: | 6 | Start page/Pages: | 1544-1548 | Source: | Journal of Infectious Diseases | Abstract: | Sera from 14 patients with duodenal ulcer and their families were tested for IgG antibodies to Helicobacter pylori. Fourteen serologically negative patients and their families served as controls. Index patients and their family members who were serologically positive were advised to undergo endoscopic biopsy. Gastric biopsy tissues were subjected to HaeIII restriction analysis of nested polymerase chain reaction products of the urease gene. By serology, 28 (49.0%) of 57 in index families and II (27.5%) of 40 in control families tested positive. A higher prevalence rate was found in children of index patients (11/31, 35.5%) than in those of control patients (1/18, 5.6%; P <.05). On DNA analysis, II patterns were found in 13 patients, and 6 families underwent endoscopy. Children in 5 families exhibited identical patterns to those of their siblings and, in 3 of the 5 families, identical to the pattern of I of the parents. These results suggest that parent-tochild transmission and common infection source are probable causes of intrafamilial clustering of H. pylori. ? 1993 by The University of Chicago. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027368079&doi=10.1093%2finfdis%2f168.6.1544&partnerID=40&md5=2b0c672397a19fee71aee90f1252368f https://scholars.lib.ntu.edu.tw/handle/123456789/417249 |
ISSN: | 0022-1899 | DOI: | 10.1093/infdis/168.6.1544 | SDG/Keyword: | dna; immunoglobulin g antibody; urease; adult; aged; article; bacterial infection; controlled study; disease transmission; duodenum ulcer; endoscopic biopsy; familial disease; female; gene amplification; helicobacter pylori; human; male; nonhuman; polymerase chain reaction; prevalence; priority journal; restriction mapping; serology; Adult; Aged; Antibodies, Bacterial; Base Sequence; Deoxyribonucleases, Type II Site-Specific; DNA, Bacterial; Duodenal Ulcer; Family Health; Female; Helicobacter Infections; Helicobacter pylori; Human; Immunoglobulin G; Male; Middle Age; Molecular Sequence Data; Polymerase Chain Reaction; Support, Non-U.S. Gov't; Urease |
Appears in Collections: | 微生物學科所 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.