|Title:||Effect of a C/EBP gene replacement on mitochondrial biogenesis in fat cells||Authors:||CHIH-HSIEN CHIU
|Keywords:||C/EBP; gene replacement; G alpha(s); WAT adipocyte; mitochondrial biogenesis||Issue Date:||15-Aug-2004||Publisher:||COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT||Journal Volume:||18||Journal Issue:||16||Start page/Pages:||1970||Source:||Genes & development||Abstract:||
CCAAT/enhancer-binding proteins, C/EBPalpha and C/EBPbeta, are required for fat cell differentiation and maturation. Previous studies showed that replacement of C/EBPalpha with C/EBPbeta, generating the beta/beta alleles in the mouse genome, prevents lipid accumulation in white adipose tissue (WAT). In this study, beta/beta mice lived longer and had higher energy expenditure than their control littermates due to increased WAT energy oxidation. The WAT of beta/beta mice was enriched with metabolically active, thermogenic mitochondria known for energy burning. The beta/beta allele exerted its effect through the elevated expression of the G protein alpha stimulatory subunit (Galphas) in WAT. Galphas, when overexpressed in fat-laden 3T3-L1 cells, stimulated mitochondrial biogenesis similar to that seen in the WAT of beta/beta mice, and effectively diminished the stored lipid pool.
|Appears in Collections:||動物科學技術學系|
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