https://scholars.lib.ntu.edu.tw/handle/123456789/425349
標題: | Effect of a C/EBP gene replacement on mitochondrial biogenesis in fat cells | 作者: | CHIH-HSIEN CHIU Lin, Wen-Der Huang, Shao-Yong Lee, Ying-Hue Lin, W.D. Huang, S.Y. |
關鍵字: | C/EBP; gene replacement; G alpha(s); WAT adipocyte; mitochondrial biogenesis | 公開日期: | 15-八月-2004 | 出版社: | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT | 卷: | 18 | 期: | 16 | 起(迄)頁: | 1970 | 來源出版物: | Genes & development | 摘要: | CCAAT/enhancer-binding proteins, C/EBPalpha and C/EBPbeta, are required for fat cell differentiation and maturation. Previous studies showed that replacement of C/EBPalpha with C/EBPbeta, generating the beta/beta alleles in the mouse genome, prevents lipid accumulation in white adipose tissue (WAT). In this study, beta/beta mice lived longer and had higher energy expenditure than their control littermates due to increased WAT energy oxidation. The WAT of beta/beta mice was enriched with metabolically active, thermogenic mitochondria known for energy burning. The beta/beta allele exerted its effect through the elevated expression of the G protein alpha stimulatory subunit (Galphas) in WAT. Galphas, when overexpressed in fat-laden 3T3-L1 cells, stimulated mitochondrial biogenesis similar to that seen in the WAT of beta/beta mice, and effectively diminished the stored lipid pool. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/425349 | ISSN: | 0890-9369 | DOI: | 10.1101/gad.1213104 |
顯示於: | 動物科學技術學系 |
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