Fluctuating reaction rate and non-exponential blinking statistics in single-enzyme kinetics
Journal
Chemical Physics Letters
Journal Volume
463
Journal Issue
1-3
Pages
134-138
Date Issued
2008
Author(s)
Abstract
Extending the Michaelis-Menten kinetic scheme, we consider a three-state diffusion-controlled reaction model to investigate the effects of fluctuating reaction rate on the blinking statistics of single-enzyme catalytic reactions. As a result of conformational changes, the barrier-height and the reaction rate for the bottleneck enzymatic reaction could fluctuate in time, leading to non-exponential blinking statistics. To illustrate model applications, some reported experimental data for single £]-galactosidase molecules were reanalyzed here to extract useful kinetic parameters. ? 2008 Elsevier B.V.
Type
journal article